300417 VO Methoden in der Zellbiologie (2012S)

Termine:May 2 to June 6, each Monday and Wednesday 1:00 3:00 pm in MFPL seminar room 1 6th floor. On June 4th it will take place on the 3rd floor, seminar room.
May 2
1. Cell clulture, protein expression and purification :
Primary cultures, organtypical cultures, differentiation models, eukaryotic expression systems, vector systems-Andreas Brachner
May 7
2.HS Struktur- Chemie B Protein-Protein, Protein DNA interactions:
In vitro binding assays, affinity (Scatchard), In situ analyses (IP), in vivo analyses, FRET, yeast two hybrid Bandshift, Footprinting, Chromatin-IP, Yeast one-hybrid Reporter Gene Assays-Andreas Brachner
May 9
3. Dynamics and Spatial Organisation of the Genome
Chromosome Conformation Capture techniques, GFP-tagging of chromosomes, High-resolution mapping ofgene territories-Alwin Köhler
May 14
4. Gene Silencing techniques RNA-Interferenz; Antisense, morpholino, epigenetics-Josef Gotzmann
May 16
5. Light Microscopy:
Immunofluorescence, wide field, confocal, live cell imaging, FRAP, FRET-Josef Gotzmann
May 21
6.
please don't turn beamer/pc off Ananlyses of cell cycle and Tumorbiology
Cell Cycle Stages (FACS, Elutriation, BrdU incorporation, proliferation marker, synchronization EMT, telomeres / DNA damage, transgenic and knock out tumor models-Roland Foisner
May 23
7. Proteomics:
Protein-identification (LC-MS/MS, mass spec, bio-informatics- Christopher Gerner
May 30
8. Techniques in Lipid Biochemistry- Sascha Martens
June 4
9. 3rd floor, seminarroom X-crystallography
Protein purification, Crystallisation, X-ray analyses, structure determination-Gang Dong
Alternative: June 6 (3rd Floor
Exam Wed., June 27 1.00pm SR 3rd floor!