301432 UE RNA-Profiling in Mikrobiologie/Immunologie (2022W)

Lehrende

• Ralf Steinborn

Termine

Pre-course briefing: Tuesday, 4rd October 2022, 15 a.m., Location: Course room of IT-Services
(Mensa- building, 2nd floor)
Course dates: Friday, 7th to Tuesday, 18th October, 2022 (Location: Course room of IT-Services,
Mensa- building, 2nd floor)
Day Time Course part Location
1 09.30 - 10.15 Introductory exam
10.15 - 11.45 Introductory lecture
11.45 - 13.00 Lunch
13.00 -15.45 Design I (Pathogen detection wit IPC)
16.00 - 17.00 Data analysis I
2 09.30 - 11.00 Lecture 2
11.00 - 12.30 Design IIa (HRM primers for CLOCK SNP)
12.30 - 13.30 Lunch
13.30 - 16.00 Design IIb (ARMS-qPCR for CLOCK SNP)
16.15 - 17:00 Session: "How to prepare good slides"
3 09.30 - 11.00 Lecture 3 (Fluorogenic dyes, Applications)
11.15 - 12.30 Data analysis II (SNP genotyping using specific primers or HRM)
12.30 - 13.30 Lunch
13.30 - 15.30 Data analysis: SNP genotyping by MCA
15.30 -17.00 Design III (RT-qPCR)
4 09.30 - 10.30 Lecture 4 (Normalisation of RT-qPCR data)
10.30 - 12.30 Data analysis III (interferon beta RNA expression induced by LPS)
12.30 - 13.30 Lunch
13.30 - 15.30 Data analysis IV (NF selection for mouse jejunum)
15.30 - 17.00 Data analysis V (circadian clock gene expression)
5 09.30 - 11.00 Lecture 5 (Digital PCR)
11.00 - 12.30 Data analysis V (Digital PCR)
12.30 - 13.30 Lunch
13.30 - 16.30 Data analysis VI (Digital PCR and statistical evaluation)
6 09.30 - 12.30 Design of Stem-loop RT-qPCR assay for miRNA quantification
12.30 - 13.30 Lunch
13.30 - 15.00 Design IVa (3 isoforms) or Design IVb (4 isoforms)
15:00 - 16.00 Training of hands-on skills to to write a research protocol
16.00 - 16.50 Excursion through Genomics Core Facility & VetCore VetCore
7 09.30 - 12.30 Preparation for Colloquium
12.30 - 13.30 Lunch
13.30 - 16.30 Colloquium IT or meadow
8 09.30 - 13.00 Presentation of technical articles
13.00 - 14.00 Lunch
14.00 - 16.00 Presentation of technical articles
IT: Course room of IT-Services (Mensa-building, 2nd floor) HOME: Protocol writing
Written test at start of course! Test is based on article by (KRALIK AND RICCHI 2017), doi:
10.3389/fmicb.2017.00108)!