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322053 VU Recombinant protein expression, purification and characterization by NMR - M14 (2024W)
Prüfungsimmanente Lehrveranstaltung
Labels
An/Abmeldung
Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").
- Anmeldung von Do 26.09.2024 12:00 bis Mo 13.01.2025 12:00
- Abmeldung bis Mo 13.01.2025 12:00
Details
Sprache: Englisch
Lehrende
Termine
20.01.2024 9:00 -15:00 @ 2F243
21.01.2024 9:00 -15:00 @ 2F243
22.01.2024 9:00 -15:00 @ 2F243
23.01.2024 9:00 -15:00 @ 2F243
24.01.2024 9:00 -15:00 @ 2F243
27.01.2024 9:00 -15:00 @ 2F243
28.01.2024 9:00 -15:00 @ 2F243
Information
Ziele, Inhalte und Methode der Lehrveranstaltung
Art der Leistungskontrolle und erlaubte Hilfsmittel
The students will be assessed on their active participation during the course of the practicum as well as through a final report.
Mindestanforderungen und Beurteilungsmaßstab
In order to attend the course, the students needs to have basic laboratory experience as well as a superficial knowledge of protein biochemistry.
Prüfungsstoff
Content of the practical
Literatur
Recombinant protein expression in Escherichia coli: advances and challengesG. L. Rosano and E. A. CeccarelliFrontiers in Microbiology 2014 Vol. 5DOI: 10.3389/fmicb.2014.00172https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2014.00172Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies. We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
Zuordnung im Vorlesungsverzeichnis
MA23
Letzte Änderung: Mo 13.01.2025 14:26
Through the course of this practicum, the students will gain an overview and hands on experience of the methods of recombinant protein expression. They will use state of the art techniques that are used in academia and industry with applications in biochemistry, structural biology, protein engineering, biotechnologies, pharmacy etc.