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270016 VO Semisynthesis of Proteins (2016S)

Methods for site-specific protein modifications by chemical and molecular biolobigy approaches

3.00 ECTS (2.00 SWS), SPL 27 - Chemie

http://www.univie.ac.at/biologischechemie1/semisynthese/

Details

Language: German

Examination dates

Lecturers

Christian Friedrich Wilhelm Becker

Classes

Freitag, 09:15-10:45 Uhr
Hörsaal IV, Halbstock, Währinger Straße 42, 1090

Information

Aims, contents and method of the course

Auxotrophie-Stämme: globaler Einbau unnatürlicher Aminosäuren, Selenomethionin

In vitro Suppression: Amber-Codon, chemisch beladene Suppressor-tRNAs, Ortsspezifität

In vivo Suppression: Orthogonales tRNA/ AaRS-Paar, Proteinevolution

Chemische Modifikation von Proteinen: Biokonjugation der proteinogenen Aminosäuren (Lys, Cys),
Oxidation N-Terminus, PLP-vermittelte Ligation, Cross-Linker

Fluoreszierende Proteine: GFP und weitere Beispiele

Chemoselektive Ligationsreaktionen: Biokonjugation artifizieller Gruppen, Oxim-Ligation, Cu(I)-katalysierte 1,3-dipolare Cycloaddition, Staudinger-Ligation, Modifikation an lebenden Zellen

Chemische Peptid-/ Proteinligation: Peptidfestphasensynthese, Sekundärstrukturbrecher, Peptidsegmentkopplung, Native Chemische Ligation, Cys-freie Ligation, Auxiliare, Inteine, Expressed Protein Ligation

Enzymatische Peptidligation: Reverse Proteolyse, Sortase, gespaltene Inteine

Assessment and permitted materials

Minimum requirements and assessment criteria

Examination topics

Reading list

Association in the course directory

BC-3, CHE II-3, CHE II-4.

Last modified: Mo 07.09.2020 15:41