Universität Wien
Warning! The directory is not yet complete and will be amended until the beginning of the term.

270028 VO Semisynthesis of Proteins (2019S)

Methods for site-specific protein modifications by chemical and molecular biolobigy approaches

3.00 ECTS (2.00 SWS), SPL 27 - Chemie

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Register/Deregister for this course

Details

Language: German

Examination dates

Lecturers

Classes (iCal) - next class is marked with N

The lecture starts on March 8!

  • Friday 01.03. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 08.03. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 15.03. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 22.03. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 29.03. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 05.04. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 12.04. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 03.05. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 10.05. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 17.05. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 24.05. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 31.05. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 07.06. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 14.06. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 21.06. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 28.06. 09:15 - 10:45 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1

Information

Aims, contents and method of the course

Informationen zur Vorlesung:
http://www.univie.ac.at/biologischechemie1/semisynthese/

Auxotrophie-Stämme: globaler Einbau unnatürlicher Aminosäuren, Selenomethionin

In vitro Suppression: Amber-Codon, chemisch beladene Suppressor-tRNAs, Ortsspezifität

In vivo Suppression: Orthogonales tRNA/ AaRS-Paar, Proteinevolution

Chemische Modifikation von Proteinen: Biokonjugation der proteinogenen Aminosäuren (Lys, Cys),
Oxidation N-Terminus, PLP-vermittelte Ligation, Cross-Linker

Fluoreszierende Proteine: GFP und weitere Beispiele

Chemoselektive Ligationsreaktionen: Biokonjugation artifizieller Gruppen, Oxim-Ligation, Cu(I)-katalysierte 1,3-dipolare Cycloaddition, Staudinger-Ligation, Modifikation an lebenden Zellen

Chemische Peptid-/ Proteinligation: Peptidfestphasensynthese, Sekundärstrukturbrecher, Peptidsegmentkopplung, Native Chemische Ligation, Cys-freie Ligation, Auxiliare, Inteine, Expressed Protein Ligation

Enzymatische Peptidligation: Reverse Proteolyse, Sortase, gespaltene Inteine

Assessment and permitted materials

Exam

Minimum requirements and assessment criteria

Examination topics

Reading list

Association in the course directory

BC-3, CHE II-3, CHE II-4

Last modified: Mo 07.09.2020 15:41