270108 VO Analytical Chemistry II (2013W)

Spectrometry: two-dimensional information, Lambert-Beer law, light source, monochromator, detector, stray light and lamp instabilities, measurement of opaque solutions, automated titrations, waveguides - evanescent field, coupling with flow injection, identification of ammonia, borate, nitrate, silicate, phosphate a.s.o.
Atomic spectroscopy: AAS - graphite tube, flame, Zeeman effect, atomisation, modifiers - matrix effects; ICP-AES - properties of plasma, dynamic range, sample preparation; applications in environmental analysis, comparison with ICP-MS;
Fluorescence: sensitivity, two dimensional techniques, quenching mechanisms; detection of oxygen, polycyclic aromatic compounds, used oils;
Photoacoustics: basic principles, depth profiles, medical applications;
FT-IR: basic principles, NIR and MIR, coupling, gas phase detection and multireflection spectroscopy, adsorption phenomena and catalysis, NIR for refuse sorting, pattern recognition;
Interfacial spectroscopy: photoelectron spectroscopy, XPS-UPS, ESCA, Auger spectroscopy,
X-ray fluorescence analysis - multielement analysis, chemical effects, matrix effects, applications: steel production, authenticity of arts products.

Contents: Fundamentals in chromatography and electrophoresis, theory and instrument setup of the most important separation methods: gas chromatography, liquid chromatography (including size exclusion, affinity and ion chromatography), capillary electrophoresis. Introduction into the theory of chromatography and electrophoresis: selectivity and efficiency. System setup of GC and LC: sample input, columns, detectors. Problem solving by GC, planar Chromatography and HPLC. Modern CE.

Contents: Mass Spectrometry - an analytical tool; instrumentation (ionization, mass analyzers, detectors, vaccuum systems); chemical structure information and fragmentation paths; data analysis; typical applications: MS coupled with separation systems (GC-MS, HPLC-MS); MS in bioanalytics and proteomics research.