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270175 UE Lab Course: Basic Techniques in Biochemistry (2020S)
for Chemistry Students
Continuous assessment of course work
Labels
Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Sa 01.02.2020 08:00 to Th 20.02.2020 18:00
- Deregistration possible until Th 20.02.2020 18:00
Registration information is available for each group.
Groups
Group 1
max. 16 participants
Language: German
LMS: Moodle
Lecturers
Classes
see german version
Aims, contents and method of the course
Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.
Group 2
max. 16 participants
Language: German
LMS: Moodle
Lecturers
Classes
see german version
Aims, contents and method of the course
Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation, isolation and restriction-analysis of plasmid DNA.
Group 3
max. 16 participants
Language: English
LMS: Moodle
Lecturers
Classes
see german version
Aims, contents and method of the course
Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation, isolation and restriction-analysis of plasmid DNA.
Group 4
max. 16 participants
Language: German
LMS: Moodle
Lecturers
Classes
see german version
Aims, contents and method of the course
Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.
Information
Assessment and permitted materials
Evaluation criteria: active participation, discussions of the experiments, experimental success, protocol and written final exam.
Minimum requirements and assessment criteria
Comprehension of basic methods in Biochemistry, planing and execution of biochemical experiments.
Active participation 50%, protocol 20%, theoretical basis and final exam 30% (the final exam must be passed with success).
Active participation 50%, protocol 20%, theoretical basis and final exam 30% (the final exam must be passed with success).
Examination topics
Content and Background of the experiments carried out during the lab course
Reading list
Course script and comprehensive theoretical supplements to the topics of the course on Moodle
Association in the course directory
BA CH 17b
Last modified: We 27.05.2020 08:49