Universität Wien
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270175 UE Lab Course: Basic Techniques in Biochemistry (2023S)

for Chemistry Students

5.00 ECTS (5.00 SWS), SPL 27 - Chemie
Continuous assessment of course work

Summary

1 Bachmair , Moodle
2 Teige , Moodle
3 Schöberl , Moodle
4 Schindler , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

max. 18 participants
Language: German
LMS: Moodle

Lecturers

Classes

Termin: 6.-17.3.2023 ganztägig
ÜR/BZB, Dr.Bohrgasse 9,1030 Wien
ANMELDUNG FÜR BIOLOGEN NUR ZU DEN KURSEN 301135, CHEMIKER NUR ZU DEN KURSEN 270175
Übungslabors 6.Stock, Biozentrum, Dr. Bohr-Gasse 9, 1030 Wien
Siehe auch Studentenseite der Institute Dr. Bohr-Gasse: http://molekularebiologie.univie.ac.at/

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

Group 2

max. 18 participants
Language: German
LMS: Moodle

Lecturers

Classes

Termin: 17. - 28.4.2023 ganztägig
ANMELDUNG FÜR BIOLOGEN NUR ZU DEN KURSEN 301135, CHEMIKER NUR ZU DEN KURSEN 270175
Übungslabors 6.Stock, Biozentrum, Dr. Bohr-Gasse 9, 1030 Wien
Siehe auch Studentenseite der Institute Dr. Bohr-Gasse: http://molekularebiologie.univie.ac.at/

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation, isolation and restriction-analysis of plasmid DNA.

Group 3

max. 18 participants
Language: German
LMS: Moodle

Lecturers

Classes

Termin: 15. - 26.5..2023 ganztägig
ANMELDUNG FÜR BIOLOGEN NUR ZU DEN KURSEN 301135, CHEMIKER NUR ZU DEN KURSEN 270175
Übungslabors 6.Stock, Biozentrum, Dr. Bohr-Gasse 9, 1030 Wien
Siehe auch Studentenseite der Institute Dr. Bohr-Gasse: http://molekularebiologie.univie.ac.at/

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation, isolation and restriction-analysis of plasmid DNA.

Group 4

max. 18 participants
Language: German
LMS: Moodle

Lecturers

Classes

Termin: 19. - 30.6.2023 ganztägig
ANMELDUNG FÜR BIOLOGEN NUR ZU DEN KURSEN 301135, CHEMIKER NUR ZU DEN KURSEN 270175
Übungslabors 6.Stock, Biozentrum, Dr. Bohr-Gasse 9, 1030 Wien
Siehe auch Studentenseite der Institute Dr. Bohr-Gasse: http://molekularebiologie.univie.ac.at/

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

Information

Assessment and permitted materials

Evaluation criteria: active participation, discussions of the experiments, experimental success, protocol and written final exam.

Minimum requirements and assessment criteria

Comprehension of basic methods in Biochemistry, planing and execution of biochemical experiments.
Active participation 50%, protocol 20%, theoretical basis and final exam 30% (the final exam must be passed with success).

Examination topics

Content and Background of the experiments carried out during the lab course

Reading list

Course script and comprehensive theoretical supplements to the topics of the course on Moodle

Association in the course directory

BA CH 17b

Last modified: Mo 08.05.2023 11:28