Universität Wien
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270190 UE Laboratory Course: Molecular Biology Techniques (2019W)

6.00 ECTS (6.00 SWS), SPL 27 - Chemie
Continuous assessment of course work
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 20 participants
Language: German

Lecturers

Classes

Verpflichtende Vorbesprechung: 2.10.2019, 9.15 Uhr
Termin: 21.10.-01.11.2019 ganztägig
Ort: ÜR/BZB, Dr.Bohrgasse 9/6.Stock, 1030 Wien.

Information

Aims, contents and method of the course

This course teaches the first steps in molecular biology research. Students learn to in vitro recombine DNA molecules (cloning), to isolate DNA from bacteria and yeast, to use PCR, and to perform bacterial transduction.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

87-100%: sehr gut
75-86.99%: gut
63-74.99%: befriedigend
50-62.99% genügend
0-49.99%: nicht genügend

Examination topics

Concentrations and dilutions, buffers, plasmids and their functional elements, plasmid extraction, PCR, restriction cloning, protein expression, SDS-PAGE, DNA (Sanger) sequencing. For methods used in the practical, it is essential to remember and understand the steps and their significance (what happens at each step). For buffers used during the practical, it is essential to understand the function of each component (remembering the exact concentration of each component is a plus, but not required).

Reading list

Association in the course directory

BIO I-1

Last modified: Sa 08.07.2023 00:21