Universität Wien
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270194 VO Immunoanalytical Methods (2023W)

2.00 ECTS (1.00 SWS), SPL 27 - Chemie
ON-SITE
Moodle

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Details

Language: German

Examination dates

Lecturers

Classes (iCal) - next class is marked with N

jeden Donnerstag, 12:00-12:45, HS 4

  • Thursday 05.10. 12:00 - 12:45 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 12.10. 12:00 - 12:45 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 19.10. 12:00 - 12:45 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 09.11. 12:00 - 12:45 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 16.11. 12:00 - 12:45 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 23.11. 12:00 - 12:45 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 30.11. 12:00 - 12:45 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 07.12. 12:00 - 12:45 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 14.12. 12:00 - 12:45 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 11.01. 12:00 - 12:45 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 18.01. 12:00 - 12:45 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 25.01. 12:00 - 12:45 Hörsaal 4 Chemie HP Währinger Straße 42

Information

Aims, contents and method of the course

Basic immunology: Immune response, structure of antibody molecules, antibody-antigen interactions, production and purification of antibodies (polyclonal, monoclonal, recombinant), characterisation of antibodies (affinity, specificity, cross reactivity), immunoaffinity chromatography;Immunoprecipitation; Immunohistochemistry;Principle of immunoassays and immunometric assays: labelling of antigens or antibodies, radioimmunoassay (tritium, I-125), enzyme immunoassay, fluoro-immunoassay, DELFIA, development and optimisation of immunoassays. Data processing: construction of the calibration curve, quality control.Lateral flow devicesImmuno-PCR

Assessment and permitted materials

oral exam, individual appointment

Minimum requirements and assessment criteria

Passing threshold at 50%50-62% "genügend", 62,5-75% "befriedigend", 75,5-88% "gut", darüber "sehr gut"

Examination topics

PowerPoint slides (Moodle)

Reading list

Wild, The Immunoassay Handbook, 2013, Elsevier

Association in the course directory

AN-1, A.5, AN-2, BC-1, CH-SAS-05

Last modified: Mo 30.09.2024 11:46