Universität Wien
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270226 PR Proteomics I (2024W)

3.00 ECTS (3.00 SWS), SPL 27 - Chemie
Continuous assessment of course work
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

Language: German

Lecturers

Classes (iCal) - next class is marked with N

Practicle course from 1st - 7th of October 2024 (max. 6 students)
Two theory blocks:
Theory block I: 1st of October 10am -12am SR3 (attendance mandatory)
Theory block II: 4rd of October 1pm - 3pm SR4
Generally, a 90% attendance at the course is mandatory.

  • Tuesday 01.10. 10:00 - 12:00 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
  • Friday 04.10. 13:00 - 15:00 Seminarraum 4 Institut Physikalische Chemie HP Währinger Straße 42

Information

Aims, contents and method of the course

The proteome of a complex sample shall be analyzed. Therefore, first, the complexity of the sample will be reduced using appropriate separation techniques. After that, an analysis of the sample, applyig mass spectrometry, will be performed. In the course of this practical work, different methods for protein analysis will be presented and practically applied.

Assessment and permitted materials

Performance is monitored during the practical course (interest, cooperation), and furthermore determined on the basis of the quality of the delivered protocol and the acquired knowledge (determined in the closing meeting).

Minimum requirements and assessment criteria

Students need positive grades for each part of the performance (interest/cooperation, protocol and closing meeting regarding the acquired knowledge) in order to successfully complete the course.

Examination topics

Background knowledge on the conducted experiments (provided in two theoretical parts and during practicle course): preparation of biological sample material, isolation of different blood constitutents or different cell types, subcellular fractionation (separation of cytoplasmic and nuclear proteins), protein separation techniques using 2D- and 1D-gel electrophoresis, digestion of proteins into petides, identification of proteins using LC-MS/MS analyses, data analysis.

Reading list

Additional literature is not required.

Association in the course directory

CH-SAS-05, BC-CHE II-1

Last modified: Tu 24.09.2024 11:46