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270281 VO Immunoanalytical Methods (2014W)

1.50 ECTS (1.00 SWS), SPL 27 - Chemie

Details

Language: German

Examination dates

Lecturers

Margit Cichna-Markl

Classes

ab Do. 09.10. von 10:00-11:00 c.t. im HS 4

Information

Aims, contents and method of the course

Basic immunology: Immune response, structure of antibody molecules, antibody-antigen interactions, production and purification of antibodies (polyclonal, monoclonal, recombinant), characterisation of antibodies (affinity, specificity, cross reactivity), immunoaffinity chromatography;
Principle of immunoassays and immunometric assays: labelling of antigens or antibodies, radioimmunoassay (tritium, I-125), enzyme immunoassay, fluoro-immunoassay, DELFIA, development and optimisation of immunoassays.
Data processing: construction of the calibration curve, quality control.
Immuno-PCR

Assessment and permitted materials

Minimum requirements and assessment criteria

Introduction into basics of immunoanalytical methods

Examination topics

Evaluation: written examination

Reading list

Association in the course directory

AN-4, 2LA-Ch 32-34, A.5;

Last modified: We 19.08.2020 08:06