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270281 VO Immunoanalytical Methods (2017W)

1.50 ECTS (1.00 SWS), SPL 27 - Chemie

Moodle

Details

max. 50 participants

Language: German

Examination dates

Lecturers

Margit Cichna-Markl

Classes (iCal) - next class is marked with N

10-11 Uhr ab 05.10.2017
Hörssaal 4

Thursday 05.10. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
Thursday 12.10. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
Thursday 19.10. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
Thursday 09.11. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
Thursday 16.11. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
Thursday 23.11. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
Thursday 30.11. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
Thursday 07.12. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
Thursday 14.12. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
Thursday 11.01. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
Thursday 18.01. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
Thursday 25.01. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42

Information

Aims, contents and method of the course

Basic immunology: Immune response, structure of antibody molecules, antibody-antigen interactions, production and purification of antibodies (polyclonal, monoclonal, recombinant), characterisation of antibodies (affinity, specificity, cross reactivity), immunoaffinity chromatography;
Immunoprecipitation; Immunohistochemistry;
Principle of immunoassays and immunometric assays: labelling of antigens or antibodies, radioimmunoassay (tritium, I-125), enzyme immunoassay, fluoro-immunoassay, DELFIA, development and optimisation of immunoassays.
Data processing: construction of the calibration curve, quality control.
Lateral flow devices
Immuno-PCR

Assessment and permitted materials

Written Exam

Minimum requirements and assessment criteria

Basics of immunoanalytical methods

Examination topics

Evaluation: written examination

Reading list

Wild, The Immunoassay Handbook, 2013, Elsevier

Association in the course directory

AN-1, AN-4, 2LA-Ch 32-34, A.5;

Last modified: Mo 07.09.2020 15:42