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300052 UE Practical Course in fluorescence-/confocal microscopy including image processing workshop (2017S)
for diploma, PhD and advanced students
Continuous assessment of course work
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Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 02.02.2017 08:00 to Th 16.02.2017 18:00
- Deregistration possible until Fr 31.03.2017 18:00
Registration information is available for each group.
Groups
Group 1
In case non-German speaking students participate we, of course, will teach in English!
max. 8 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
Preliminary Meeting: Thu, March 2nd, 11.00am-11.30am (attendance mandatory)
Sem room 3, 6th floor, MFPL
In case of overbooking the head of the course will selcet people on the basis of career status and the demand on performing microscopic experiments in the course of a Master/PhD-thesis in a group.
Seat limitation: 8 (per course; in total 16)
More schedule and programme details at the preliminary meeting.Dates: all days are full-day (unless otherwise noted)
Course1: March, 14,15,16,21,23; April 4,5 (Mandatory attendance at all days)
Course 2: March 14+15 (9-12.30 only); March 16,28,29,30; April 4+5 (Mandatory attendance at all days)Location: VBC5, MFPL, Dr. Bohrg.9, 1030 Vienna
Assessment and permitted materials
oral exam and continuous assessment
Group 2
In case non-German speaking students participate we, of course, will teach in English!
max. 8 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
Preliminary Meeting: Thu, March 2nd, 11.00am-11.30am (attendance mandatory)
Sem room 3, 6th floor, MFPL
In case of overbooking the head of the course will selcet people on the basis of career status and the demand on performing microscopic experiments in the course of a Master/PhD-thesis in a group.
Seat limitation: 8 (per course; in total 16)
More schedule and programme details at the preliminary meeting.Dates: all days are full-day (unless otherwise noted)
Course1: March, 14,15,16,21,23; April 4,5 (Mandatory attendance at all days)
Course 2: March 14+15 (9-12.30 only); March 16,28,29,30; April 4+5 (Mandatory attendance at all days)Location: VBC5, MFPL, Dr. Bohrg.9, 1030 Vienna
Assessment and permitted materials
oral exam and continuous assessmen
Information
Aims, contents and method of the course
Minimum requirements and assessment criteria
The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.
Examination topics
microscopy sample preparation
widefield fluorescence microscopy (incl. Köhler and contrast enhancement)
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software
widefield fluorescence microscopy (incl. Köhler and contrast enhancement)
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software
Reading list
J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006
Yuste, Imaging – A Laboratory manual; CSHL-Press, 2010
D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009
E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009
Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008
Yuste, Imaging – A Laboratory manual; CSHL-Press, 2010
D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009
E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009
Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008
Association in the course directory
PhD-MB 2, MMEI III, MMB W-2, WZB
Last modified: Mo 07.09.2020 15:43
Quality criteria for dealingwith aberrations correctionpractical microscopy of selected samples by fluorescence- and confocal microscopy (multichannel detection, serial optical sections through samples, image projections, generation of time series)image analysis and deconvolution of selected images