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300073 UE Modern Light Microscopy in Study and Research (2024W)
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 12.09.2024 14:00 to Th 26.09.2024 18:00
- Deregistration possible until Tu 15.10.2024 18:00
Details
max. 12 participants
Language: German, English
Lecturers
Classes (iCal) - next class is marked with N
Registration, preliminary discussion (compulsory attendance) AND 1st teaching unit on Friday, 4 October 2024 at 16:00 - 18:00, seminar room 4.1 (4th floor, lift near staircase 1).
Discussion of the dates for > questions about the material (presumably 28 or 29 Oct.), the > theoretical exam (presumably 8 Nov.) and the > blocked internship (presumably in the week 20-23 Nov.) as well as the > presentation date (about 2 weeks later).
Last possible deregistration: 4 October 2024, as the first lesson already starts here.
- Friday 13.12. 16:45 - 18:15 Seminarraum 1.2, Biologie Djerassiplatz 1, 1.004, Ebene 1
Information
Aims, contents and method of the course
Assessment and permitted materials
Attendance is compulsory, a maximum of 1 day can be missed due to illness.
The preliminary meeting is considered to be the first teaching unit and is therefore subject to compulsory attendance! If registered students are absent from the preliminary meeting without prior agreement (email!), the course instructor will cancel the course.
Since the preliminary meeting is considered the first course unit and thus course participation has begun, deregistration after the deregistration deadline (15.10.2024) is only possible in justified exceptional cases (e.g. doctor's confirmation). If no sufficiently substantiated justification is provided, this exercise must be assessed negatively.
A single excused absence (8 hours) during the exercise weeks is permitted. Excusable reasons are, for example, a doctor's appointment with confirmation or compulsory attendance at other courses or examinations.
The performance assessment consists of three parts
- Written examination on the theoretical part (weighting 40%)
- Active participation during the hands-on internship (weighting 20%)
- Final presentation on the last day of the course or on a day agreed in the preliminary discussion (weighting 40%)
For work in the microscopy laboratory in the Core Facility (CIUS), the internal safety course must be completed, this counts towards the total hours. It is mandatory to bring a lab coat on the days when samples are prepared. It is assumed that previous experience with course microscopes has already been gained in previous practical courses and that laboratory experience and computer skills are available.
The preliminary meeting is considered to be the first teaching unit and is therefore subject to compulsory attendance! If registered students are absent from the preliminary meeting without prior agreement (email!), the course instructor will cancel the course.
Since the preliminary meeting is considered the first course unit and thus course participation has begun, deregistration after the deregistration deadline (15.10.2024) is only possible in justified exceptional cases (e.g. doctor's confirmation). If no sufficiently substantiated justification is provided, this exercise must be assessed negatively.
A single excused absence (8 hours) during the exercise weeks is permitted. Excusable reasons are, for example, a doctor's appointment with confirmation or compulsory attendance at other courses or examinations.
The performance assessment consists of three parts
- Written examination on the theoretical part (weighting 40%)
- Active participation during the hands-on internship (weighting 20%)
- Final presentation on the last day of the course or on a day agreed in the preliminary discussion (weighting 40%)
For work in the microscopy laboratory in the Core Facility (CIUS), the internal safety course must be completed, this counts towards the total hours. It is mandatory to bring a lab coat on the days when samples are prepared. It is assumed that previous experience with course microscopes has already been gained in previous practical courses and that laboratory experience and computer skills are available.
Minimum requirements and assessment criteria
The course is assessed positively (grade 4) if 55% of the possible points are achieved in both the written examination and the final presentation (20 min speaking time for the group + 5 min discussion). Each partial performance is assessed separately and is included in the overall assessment of the course in different percentages (see above).
Examination topics
Will be discussed as part of the first unit.
Reading list
Web pages of different microscope manufactures are highly recommended: e.g. MicroscopyU, (Nikon 2018), also other manufacturers: Zeiss, Olympus, Leica;Oldfield R., 1994: Light Microscopy: an Illustrated Guide. Wolfe Publishing. ISBN 0 7234 1876 4 Slayter, E.M., Slayter, H.S., 1992: Light and Electron Microscopy. Cambridge University PressBradbury, S., Bracegirdle, B., 1998: Introduction to Light Microscopy. Royal Microscopical Society Microscopy Handbooks 42. BIOS Scientific Publishers Ltd, Oxford, UK. ISBN 1 859961 21Bradbury, S., Evenett, P.J., 1996: Contrast Techniques in Light Microscopy. Royal Microscopical Society Microscopy Handbooks 34. BIOS Scientific Publishers Ltd, Oxford, UK.ISBN 1 85996 085 5Fluorescence Microscopy. From Principles to Biological Applications (2017) Edited by Ulrich Kubitscheck, Wiley, 482 p e-book (online via u:access)
Association in the course directory
MBO 5, MZO2, UF MA BU 04
Last modified: Tu 03.12.2024 15:26
The theoretical content will be presented in the first unit and will be partly developed independently, using literature and video lessons. In a further session, the microscopes will be presented on site and you will be able to ask questions about the material covered in a ‘flipped classroom’ format:
- Structure of a microscope and the individual components: Objective types, beam path, resolution & illumination.
- Presentation of various contrast methods (bright field, dark field, phase contrast, DIC, polarization, fluorescence).
The written examination concludes this part and is intended to ensure that knowledge can be deepened in the practical part.
In the subsequent practical part, participants gain an overview of the techniques and in particular the possibilities for measurement and documentation on the light microscopes in the UBB Core Facility (stereo, transmitted light, fluorescence and laser scanning microscopy). You will work on samples in small groups with all types of microscopes and communicate the results to all participants in a PowerPoint presentation to reinforce the memory effect.
As a result of the course, you should be able to select the correct and most efficient method of light microscopy for your own sample and research question.