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300190 PP Hands-on Cryotechniques for Immuno-Electron Microscopy and Tissue in-situ Hybridization (2011W)
To enable advanced BIO-stud. to understand & apply advan.cryo-electron microscopy, immunolabelling & tissue in-situ hybridization (TISH) tech. within their own research. Supported by lectures & demonst., this intensive, hand-on training courese teaches special. techn.,such as Tokuyasu cryosectioning, high-pressure freezing-freeze substitution & acrylic resin embedding for high resol. localization of intracell. proteins & other intracell. struc. by immunogold labelling or TISH
Continuous assessment of course work
Labels
Hands-on Cryotechiques in immunoEM and ISH courseAll lectures will take place in the Seminar Room 1 UZA I (Zoology Dept.)Datum N.U: 07.10.2011
Zeit: 0900-1100 Preliminary lecture
Ort: UZA I Seminar Room 114.11.11 0900-1300 First Lectures;
16.11.11 0900-1100 (Student talks); 17.11.11 0900-1100; 22.11.11 0900-1100; 24.11.11 0900-1100; 25.11.11 0900-1100; 28.11.11 0900- 1100; 29.11.11 0900-1000; 30.11.11 0900-1100; 02.12.11 0900-1100 Final Lecture (Students results talks).Practical sessions will be held in Ultrastructure Research Laboratory, Electron Microscopy Lab in Althanstrasse 14, 1090; 14.11.11 0900- 1700 each day for three weeks until 02.12.11 0900- 1700 inclusive, when there is no lecture, the practical session may begin earlier, but you will be advised. ; Additional practical sessions will be held in Electron Microscopy Lab, Dr.Bohrgasse 9, 1030 and also in Electron Microscopy Lab of IMBA, Dr. Bohrgasse 6, 1030.All students are requested to provide an abstract (max 300 words) describing your major research interest and the intended application of immuno-electron microscopy and/or tissue in-situ hybridization in your research. This abstract must be received by email to Dr. Jacqueline Montanaro and Dr. Siegfried Reipert. Latest acceptance of abstract is 14. October, 2011.In addition, please provide the following information:; Your Name, Institute, Department, Phone number, email, webpage and please indicate (yes or no) whether you have previous experience with ; LRW embedding, FISH, TEM, Epifluorescence microscopy, cryo-microtomy and any immuno-staining techniques at light microscopy or electron microscopy level.; ; Please send abstracts and further information to:; jacqueline.montanaro@meduniwien.ac.at and siegfried.reipert@univie.ac.at.You are advised that you will be required to give a 5 minute introductory talk during the course (17.11.09) describing your major research interest and the intended application of immuno- electron microscopy and/or tissue in-situ hybridization in your research. This is intended to be an introduction of the students to the lecturers and to eachother. This talk will also contribute towards the final assessment.
Zeit: 0900-1100 Preliminary lecture
Ort: UZA I Seminar Room 114.11.11 0900-1300 First Lectures;
16.11.11 0900-1100 (Student talks); 17.11.11 0900-1100; 22.11.11 0900-1100; 24.11.11 0900-1100; 25.11.11 0900-1100; 28.11.11 0900- 1100; 29.11.11 0900-1000; 30.11.11 0900-1100; 02.12.11 0900-1100 Final Lecture (Students results talks).Practical sessions will be held in Ultrastructure Research Laboratory, Electron Microscopy Lab in Althanstrasse 14, 1090; 14.11.11 0900- 1700 each day for three weeks until 02.12.11 0900- 1700 inclusive, when there is no lecture, the practical session may begin earlier, but you will be advised. ; Additional practical sessions will be held in Electron Microscopy Lab, Dr.Bohrgasse 9, 1030 and also in Electron Microscopy Lab of IMBA, Dr. Bohrgasse 6, 1030.All students are requested to provide an abstract (max 300 words) describing your major research interest and the intended application of immuno-electron microscopy and/or tissue in-situ hybridization in your research. This abstract must be received by email to Dr. Jacqueline Montanaro and Dr. Siegfried Reipert. Latest acceptance of abstract is 14. October, 2011.In addition, please provide the following information:; Your Name, Institute, Department, Phone number, email, webpage and please indicate (yes or no) whether you have previous experience with ; LRW embedding, FISH, TEM, Epifluorescence microscopy, cryo-microtomy and any immuno-staining techniques at light microscopy or electron microscopy level.; ; Please send abstracts and further information to:; jacqueline.montanaro@meduniwien.ac.at and siegfried.reipert@univie.ac.at.You are advised that you will be required to give a 5 minute introductory talk during the course (17.11.09) describing your major research interest and the intended application of immuno- electron microscopy and/or tissue in-situ hybridization in your research. This is intended to be an introduction of the students to the lecturers and to eachother. This talk will also contribute towards the final assessment.
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 01.09.2011 08:00 to We 21.09.2011 20:00
- Deregistration possible until Su 30.10.2011 23:00
Details
max. 6 participants
Language: English
Lecturers
Classes
Currently no class schedule is known.
Information
Aims, contents and method of the course
Fixation of (own) material with the help of high pressure freezing and freeze substitution methods; embedding for investigation of ultrastructure or for immunolabelling; preparation of semithin and ultrathin sections; introduction to the Tokuyasu method for preparing cryosections and applying immunogold labelling. Tissue in situ hybridization (TISH) on acryl semithin sections. Evaluation and detailed discusssion of results at the transmission electron microscope and fluorescence microscope.
Assessment and permitted materials
Student assessment is divided into 4 parts: Introductory Student talk (5 mins), Participation in lectures and practicum, Individual oral assessment of understanding technical and theoretical knowledge presented in lectures and demonstrations (end of course), Final student talk (7-10 mins) followed by open discussion and questions.
Minimum requirements and assessment criteria
To enable advanced BIO-stud. to understand & apply advan.cryo-electron microscopy, immunolabelling & tissue in-situ hybridization (TISH) tech. within their own research. Supported by lectures & demonst., this intensive, hand-on training course teaches special. techn.,such as Tokuyasu cryosectioning, high-pressure freezing-freeze substitution & acrylic resin embedding for high resol. localization of intracell. proteins & other intracell. struc. by immunogold labelling or TISH
Examination topics
---To enable advanced BIO-stud. to understand & apply specialized advanced Tokuyasu cryosectioning, high pressure freezing, freeze substitution and acrylic resin embedding as preparation techniques for light microscopy (LM) and electron microscopy (EM). These advanced processing techniques prepare for gene/ protein of interest labelling with tissue in-situ hybridization (TISH) and immunolabelling by immunogold and immunofluorescence. In addition, the course is complemented by excursions to off-site centres of excellence in EM techniques not offered at Althanstrasse. Supported by lectures & demonst., this intensive, hand-on training course teaches advanced LM and EM preparation and immunolabeling techniques for high resol. localization of intracell. proteins & other intracell. structures. All these techniques are applied under expert guidance to further advance the students own research at both LM and EM level.
Reading list
Association in the course directory
MMB W-2, MMI III-2, MGE III-2, MZO 2, MEV 2, MPF W-2
Last modified: Mo 07.09.2020 15:43