Universität Wien

300218 UE Scanning electron microscopy - techniques and preparations for biologists (2022W)

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
ON-SITE
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 6 participants
Language: German, English

Lecturers

Classes (iCal) - next class is marked with N

Online preliminary meeting 21.11.2022 10:00. I will send the link in time.
The lecture for the course will take place on December 1st, 2022 in seminar room 1.1 PC.
Sample preparation will take place for everyone on December 2nd, 2022 and December 6th, 2022 in the EM laboratory of the Core Facility Cell Imaging and Ultrastructural Research (attendance required).
Training on the two scanning electron microscopes and energy-dispersive X-ray microanalysis will take place on December 7th, 2022 and December 9th, 2022. (Mandatory attendance)
From December 13th to 15th, free work will take place on the scanning electron microscopes. You will be able to choose time slots for these 3 days in the preliminary meeting.
On 16.12. the final meeting of the course takes place (attendance is compulsory).

If Corona measures prevent the simultaneous presence of all course participants on our lecture rooms and lab rooms, the lecture and the final meeting will take place online and the practical part in individual lessons (details on this in the preliminary meeting).

  • Thursday 01.12. 08:00 - 14:45 Seminarraum 1.1 PC, Biologie Djerassiplatz 1, 1.003, Ebene 1

Information

Aims, contents and method of the course

Contents and methods:
Our Scanning Electron Microscopes (SEM) offer various techniques to investigate samples.
In this course you will have the opportunity to learn the necessary preparation steps and the usage of the SEM in several modes so that you can apply these techniques with your own samples afterwards.
On the SEM you will use the following modes:
Secondary electron detector in low vacuum and high vacuum, backscatter electron detector in low vacuum and high vacuum, energy dispersive x-ray micro analysis with EDX detector.

Assessment and permitted materials

Choice of relevant methods for sample preparation, work in our chemlab, operating preparation instruments and SEM, evaluation and interpretation of results.
1) Moodle Test - Safety instructions for the EM Lab (has to be done before practical work)
2) Cooperation
3) Short Presentation
4) Worksheets

Minimum requirements and assessment criteria

Every part has to be positive
Safety instructions 25 points
Cooperation 25 points
Short presentation 25 points
Worksheets 25 points

0 - 50 points 5
51 - 67 points 4
67 - 81 points 3
82 - 90 points 2
91 - 100 points 1

Examination topics

Theoretical background (lecture). Operating the preparation instruments and the SEM (practical work). Evaluation and interpretation of the results.

Reading list

Will be presented during the course.

Association in the course directory

M-WZB, MAN 3, MEC-9, MBO 7, MZO 2, MZO4, MZO2

Last modified: Mo 23.01.2023 14:09