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300239 UE Multiple preparation techniques for use in light- and electron microscopy (2024W)
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 12.09.2024 14:00 to Th 26.09.2024 18:00
- Deregistration possible until Tu 15.10.2024 18:00
Details
max. 10 participants
Language: German, English
Lecturers
Classes (iCal) - next class is marked with N
Preliminary meeting: 02.10.2024, 10:00-11:00 a.m. Place: Department of Botany and Biodiversity Research, Rennweg 14, 1030 Wien. If you cannot be there, please send a representative. The course will be held in a blocked form over five days from 10.02-14.02.2025, between 09-18:00 at Rennweg 14.
- Wednesday 02.10. 10:00 - 11:00 Übungsraum 1 (Fakultätszentrum für Biodiversität) Rennweg 1.OG
- N Monday 10.02. 09:00 - 18:00 Übungsraum 1 (Fakultätszentrum für Biodiversität) Rennweg 1.OG
- Tuesday 11.02. 09:00 - 18:00 Übungsraum 1 (Fakultätszentrum für Biodiversität) Rennweg 1.OG
- Wednesday 12.02. 09:00 - 18:00 Übungsraum 1 (Fakultätszentrum für Biodiversität) Rennweg 1.OG
- Thursday 13.02. 09:00 - 18:00 Übungsraum 1 (Fakultätszentrum für Biodiversität) Rennweg 1.OG
- Friday 14.02. 09:00 - 18:00 Übungsraum 1 (Fakultätszentrum für Biodiversität) Rennweg 1.OG
Information
Aims, contents and method of the course
Assessment and permitted materials
Based on a written protocol (60%) and active participation (40%) throughout the course.
Minimum requirements and assessment criteria
Students prepare specimens for LM (Kulzer-embedding, microtomy, different staining techniques) and SEM/TEM, they document specimens digitally, analyse, and interpret their own results.
Examination topics
Demonstrations, and practical work throughout the course.
Reading list
BEHRENS W (1883) Hilfsbuch zur Ausführung mikroskopischer Untersuchungen im botanischen Laboratorium. Schwetschke & Sohn, Braunschweig. 387 pp.
BÖCK P (1984) Der Semidünnschnitt. - Bergmann, München. 172 pp.
GERLACH D (1984) Botanische Mikrotechnik. 3. Aufl., Thieme: Stuttgart, New York. 311 pp.
BÖCK P (1984) Der Semidünnschnitt. - Bergmann, München. 172 pp.
GERLACH D (1984) Botanische Mikrotechnik. 3. Aufl., Thieme: Stuttgart, New York. 311 pp.
Association in the course directory
MBO 5, MEC-9, MZO 2
Last modified: We 25.09.2024 11:07
• Microtomy (sectioning, 5 µm thin)
• Fixation (application of different methods and comparison)
• Staining techniques (toluidine blue/ruthenium red, acetolysis)
• Light microscopy (LM)
• Scanning electron microscopy (SEM)
• Transmission electron microscopy (TEM)