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300286 UE Fluorescence in situ hybridization (FISH) (2024W)
Identification of uncultured microorganisms
Continuous assessment of course work
Labels
Für diese LV wird eine verpflichtende Sicherheitsunterweisung stattfinden. Nach Ablauf der Anmeldephase und Aufnahme der Studierenden, muss diese absolviert werden. Der link zum entsprechenden Moodle-Kurs wird ihnen nach Aufnahme in den Kurs zugesendet.
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
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Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
_
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 12.09.2024 14:00 to Th 26.09.2024 18:00
- Deregistration possible until Tu 15.10.2024 18:00
Details
max. 12 participants
Language: German
Lecturers
Classes (iCal) - next class is marked with N
Blocked course: 3.2. - 14.2.2025
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N
Monday
03.02.
08:00 - 18:15
Seminarraum 2.1, Biologie Djerassiplatz 1, 2.129, Ebene 2
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1 -
Tuesday
04.02.
08:00 - 18:15
Seminarraum 2.1, Biologie Djerassiplatz 1, 2.129, Ebene 2
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1 -
Wednesday
05.02.
08:00 - 18:15
Seminarraum 2.1, Biologie Djerassiplatz 1, 2.129, Ebene 2
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1 -
Thursday
06.02.
08:00 - 18:15
Seminarraum 2.1, Biologie Djerassiplatz 1, 2.129, Ebene 2
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1 -
Friday
07.02.
08:00 - 18:15
Seminarraum 2.1, Biologie Djerassiplatz 1, 2.129, Ebene 2
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1 - Monday 10.02. 08:00 - 18:15 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
- Tuesday 11.02. 08:00 - 18:15 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
- Wednesday 12.02. 08:00 - 18:15 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
- Thursday 13.02. 08:00 - 18:15 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
- Friday 14.02. 08:00 - 18:15 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Information
Aims, contents and method of the course
In this laboratory course, prokaryotes in environmental and medical samples will be detected and identified using the full cycle rRNA approach and FISH with rRNA-targeted oligonucleotide probes. The experiments performed will provide in-depth insights into parameters relevant for nucleic acid hybridization techniques and will expose students to epifluorescence- and confocal laser scanning microscopy. In addition, attendants will learn how to apply digital image analysis to extract quantitative data from FISH experiments.
Assessment and permitted materials
Continuous assessment of course work. Participation during the course, quizes, written protocol, seminar presentation.
Minimum requirements and assessment criteria
Presence at all course dates.
All components (active participation, quiz, seminar presentation, written protocol) must be passed.
All components (active participation, quiz, seminar presentation, written protocol) must be passed.
Examination topics
Content of lectures during the course and content of course hand book
Reading list
A comprehensive course guide will be provided; additional resources are documents provided with the lectures and the seminar.
Association in the course directory
BOE 12, BMB 10, BMG 10
Last modified: Mo 26.08.2024 17:06