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300306 UE Hands-on Immunolabelling for Light and Electron Microscopy (2017W)
techniques and preparations for biologists
Continuous assessment of course work
Labels
Meeting is compulsory, but students can also send representative in their place.For further questions, please send email to jacqueline.montanaro@univie.ac.at
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 07.09.2017 08:00 to Th 21.09.2017 18:00
- Deregistration possible until Tu 31.10.2017 18:00
Details
max. 5 participants
Language: English
Lecturers
Classes
Datum: 30.10.2017
Monday 30.10.2017; 13:30-15:00, Seminar Room 1 UZA1, (verpflichtende Teilnahme).
Meeting is compulsory, but students can also send representative in their place.
Practical work from 12:30 6.11.2017 until Monday 13.11.2017 16:00 inclusive., EM-LAB Core Facility Cell Imaging and Ultrastructure Research UZA1
Final discussion: (Tuesday) 14.11.2017, 08:30-12:00, Seminar Room 1 UZA1Presentation of Protocol-Poster:
Tuesday 28th November, 17:00 – 20:30, Zoologie Konference Raum, UZA1For further questions, please send email to jacqueline.montanaro@univie.ac.atPlease note: Block course 06.11.2017 until 14.11.2017 inclusive
Information
Aims, contents and method of the course
The goal of the course is to teach Masters and PhD students about different methods which can be used to prepare and perform immunolabelling at light and electron microscopy level, including how to prepare semi-thin and ultrathin sections using a Cryoultramicrotome (Tokuyasu method). Immunolabelling and contrasting using negative staining, cryosections and acrylic sections will be evaluated and compared using the fluorescence and transmission electron microscopes. Finally, stereological methods that may be useful for quantification of immunogold labelling will be presented and discussed.
Assessment and permitted materials
Participation: 40
Oral Exam: 30
Protocol or Poster: 30
Performance in each part must be positive
Oral Exam: 30
Protocol or Poster: 30
Performance in each part must be positive
Minimum requirements and assessment criteria
as above
Examination topics
Knowledge of the theoretical background (lectures). Application of the technical aspects of immunolabeling for LM and TEM (from the practical part). Applications in examples. Analysis, interpretation and evaluation of results.
Reading list
Will be discussed during the Practical Instruction
Association in the course directory
MZO W-1, MBO 7, MMB W-2, MGE III-2, MEV 2,
Last modified: Mo 07.09.2020 15:43