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300306 UE Hands-on Immunolabelling for Light and Electron Microscopy (2018W)
techniques and preparations for biologists
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 06.09.2018 08:00 to Th 20.09.2018 18:00
- Deregistration possible until We 31.10.2018 18:00
Details
max. 5 participants
Language: English
Lecturers
Classes
First date: Thursday 25 .10.2018; 13.30 pm, Seminar Room 1, UZA1. Meeting is compulsory, but students can also send representative in their place.
Summary: Block course 05.11.2018 until 14.11.2018 inclusive; (Attention: the laboratory is not available on 12.11.2018, therefore no course will take place on this day); plus one extra evening meeting.Details Block course: 5-14 November 2018 (except 12.11.2018); inclusive 08.30-16:30 each dayMo. 05.11.2018 08:30 – 12:30 Seminar Room 1 UZA1, 13:30 – 16:30 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research. Practical work continues in EM lab.
Results discussion Tuesday 13th November, 08:30-12:00 Seminar Room 1, UZA1
Note: approx. 2 weeks after last course day, allow for additional evening meeting for group oral exam; student presentations; exact day to be arranged at preliminary meeting.Questions: please send email to jacqueline.montanaro@univie.ac.at
Information
Aims, contents and method of the course
The goal of the course is to teach Masters and PhD students about different methods which can be used to prepare and perform immunolabelling at light and electron microscopy level, including how to prepare semi-thin and ultrathin sections using a Cryoultramicrotome (Tokuyasu method). Immunolabelling and contrasting using negative staining, cryosections and acrylic sections will be evaluated and compared using the fluorescence and transmission electron microscopes. Finally, stereological methods that may be useful for quantification of immunogold labelling will be presented and discussed.
Assessment and permitted materials
Participation: 40
Oral Exam: 30
Protocol or Poster: 30
Performance in each part must be positive
Oral Exam: 30
Protocol or Poster: 30
Performance in each part must be positive
Minimum requirements and assessment criteria
as above
Examination topics
Knowledge of the theoretical background (lectures). Application of the technical aspects of immunolabeling for LM and TEM (from the practical part). Applications in examples. Analysis, interpretation and evaluation of results.
Reading list
Wird während des Praktikums bekannt gegeben
Association in the course directory
MZO W-1, MBO 7, MMB W-2, MGE III-2,
Last modified: Fr 31.08.2018 08:43