Universität Wien

300306 UE Hands-on Immunolabelling for Light and Electron Microscopy (2023W)

Ultrastructure preservation for biologists

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
ON-SITE

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 8 participants
Language: English

Lecturers

Classes (iCal) - next class is marked with N

03.11.2023 Please confirm your participation by email.
(if necessary, please deregister in time to give students on waiting list a chance to attend the course)
03.11.2023 You will get the official link to the Moodle course "Safety Instructions for the Core Facility Cell Imaging and Ultrastructure Research". Please run the test of the Moodle course. This will be available until 10.11.2023
10.11.2023. Official start of the course. Attendance on this day is compulsory, but you can send someone in your place if necessary.
Please note: This is an intensive 2 week hands-on course.
13-11-2023 Lectures Part 1. 08:00 - 14:45 General introduction to Transmission Electron Microscopy, Fixation, Immunolabeling.
14.11-2023 Sample preparation /Introduction to Ultramicrotome
15.11.2023 Sample preparation /Introduction to Ultramicrotome
16.11.2023 Immunolabeling / Ultramicrotome
17.11.2023 Immunolabeling / Ultramicrotome
20.11.2023 Lectures Part 2. 09:45 - 11:15 Understanding your samples in the TEM
20.22.2023 12.30-16:30 Introduction to TEM (4 x 1 hour timeslots)
21.11.2023 Ultramicrotome/TEM timeslots*
22.11.2023 Ultramicrotome/TEM timeslots*
23.11.2023 Ultramicrotome/TEM timeslots*
24.11.2023 Visit to ISTA, Klosterneuburg. Lecture and tour Electron Microscopy Facility
*Allocation of timeslots will be done at beginning of course
Approx. 2 weeks after completion of course, a mutually agreed meeting time will be arranged for oral exam and presentation of poster/protocols.

  • Friday 03.11. 13:15 - 14:45 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
  • Friday 10.11. 13:15 - 14:45 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
  • Monday 13.11. 08:00 - 14:45 Seminarraum 1.1 PC, Biologie Djerassiplatz 1, 1.003, Ebene 1
  • Monday 20.11. 09:45 - 11:15 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1

Information

Aims, contents and method of the course

The goal of the course is to teach Masters and PhD students about different methods which can be used to prepare and perform routine electron microscopy (EM), including how to prepare semi-thin and ultrathin sections using an ultramicrotome and an introduction to the transmission electron microscope. In addition, immunolabelling and contrasting using negative staining and acrylic sections will be evaluated and compared. Finally, stereological methods that may be useful for quantification of immunogold labelling will be presented and discussed. Advanced methods for sample preparation and other label detection methods will be discussed. Students participating in this course will gain a good understanding of routine and immunological methods for transmission EM. In addition, students can gain practical experience in preparing samples for electron microscopy and also how to interpret both routine preparations for transmission electron microscopy and how to interpret results of immunolabelling experiments.

Assessment and permitted materials

Each part must be positive:
Safety training: 10 points
Cooperation: 20 points
Short presentation: 25 points
Detailed protocol or poster: 25 points
Oral examination: 20 points

0 - 50 points 5
51 - 67 points 4
67 - 81 points 3
82 - 90 points 2
91 - 100 points 1

Minimum requirements and assessment criteria

as above

Examination topics

Knowledge of the theoretical background (lectures); Safety in the electron microscopy lab. Understanding of different preparation methods. Applications of the technical aspects of immunolabeling for LM and TEM (from the practical part). Analysis, interpretation and evaluation of results.

Reading list

Will be announced during the practical course

Association in the course directory

MZO4, MBO 7, MMB W-2, MGE III-2, MAN 3, MEC-9, MZO2, CoBeNe 3

Last modified: Tu 15.08.2023 10:27