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300316 PP Fluorescence in situ Hybridization (FISH) (2009W)
Continuous assessment of course work
Labels
Ersttermin ist Vorbesprechungstermin!Prüfung am 22.2.2009, 10 UhrRegistrierung für den Zugriff auf LV-relevante Unterlagen: http://www.microbial-ecology.net/education
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 10.09.2009 10:00 to Su 27.09.2009 23:00
- Deregistration possible until Fr 30.10.2009 23:00
Details
max. 12 participants
Language: German
Lecturers
Classes (iCal) - next class is marked with N
- Monday 08.02. 09:00 - 17:00 Schreibraum/Seminarraum
- Tuesday 09.02. 09:00 - 16:55 Schreibraum/Seminarraum
- Wednesday 10.02. 09:00 - 16:55 Schreibraum/Seminarraum
- Thursday 11.02. 09:00 - 16:55 Schreibraum/Seminarraum
- Friday 12.02. 09:00 - 16:55 Schreibraum/Seminarraum
- Monday 15.02. 09:00 - 16:55 Schreibraum/Seminarraum
- Tuesday 16.02. 09:00 - 16:55 Schreibraum/Seminarraum
- Wednesday 17.02. 09:00 - 16:55 Schreibraum/Seminarraum
- Thursday 18.02. 09:00 - 16:55 Schreibraum/Seminarraum
- Friday 19.02. 09:00 - 16:55 Schreibraum/Seminarraum
Information
Aims, contents and method of the course
This laboratory course will teach attendants how to specifically detect and identify prokaryotes in environmental and medical samples using the full cycle rRNA approach and FISH with rRNA-targeted oligonucleotide probes. The experiments performed will provide in-depth insights into parameters relevant for nucleic acid hybridization techniques and will expose students to epifluorescence- and confocal laser scanning microscopy. In addition, attendants will learn how to apply digital image analysis to extract quantitative data from FISH experiments.
Assessment and permitted materials
Continuous assessment of course work. Quality of experimental work, seminar presentation; participation in the seminar discussion, qualitiy of protocol and written exam
Minimum requirements and assessment criteria
Acquisition of methods to extract DNA from environmental samples, to PCR-amplify and clone specific genes, and of FISH and fluorescence microscopy. Use of these methods on participants' own environmental samples and evaluation of the results.
Examination topics
Laboratory experiments in groups of two, introduction and discussion of the theoretical background in lectures and seminars
Reading list
Association in the course directory
BOE 10, BOE 11, BOE 12, MMI I-2
Last modified: Sa 22.10.2022 00:30