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300417 VO Methods in Cell Biology (2012S)
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Termine:May 2 to June 6, each Monday and Wednesday 1:00 3:00 pm in MFPL seminar room 1 6th floor. On June 4th it will take place on the 3rd floor, seminar room.
May 2
1. Cell clulture, protein expression and purification :
Primary cultures, organtypical cultures, differentiation models, eukaryotic expression systems, vector systems-Andreas Brachner
May 7
2.HS Struktur- Chemie B Protein-Protein, Protein DNA interactions:
In vitro binding assays, affinity (Scatchard), In situ analyses (IP), in vivo analyses, FRET, yeast two hybrid Bandshift, Footprinting, Chromatin-IP, Yeast one-hybrid Reporter Gene Assays-Andreas Brachner
May 9
3. Dynamics and Spatial Organisation of the Genome
Chromosome Conformation Capture techniques, GFP-tagging of chromosomes, High-resolution mapping ofgene territories-Alwin Köhler
May 14
4. Gene Silencing techniques RNA-Interferenz; Antisense, morpholino, epigenetics-Josef Gotzmann
May 16
5. Light Microscopy:
Immunofluorescence, wide field, confocal, live cell imaging, FRAP, FRET-Josef Gotzmann
May 21
6.
please don't turn beamer/pc off Ananlyses of cell cycle and Tumorbiology
Cell Cycle Stages (FACS, Elutriation, BrdU incorporation, proliferation marker, synchronization EMT, telomeres / DNA damage, transgenic and knock out tumor models-Roland Foisner
May 23
7. Proteomics:
Protein-identification (LC-MS/MS, mass spec, bio-informatics- Christopher Gerner
May 30
8. Techniques in Lipid Biochemistry- Sascha Martens
June 4
9. 3rd floor, seminarroom X-crystallography
Protein purification, Crystallisation, X-ray analyses, structure determination-Gang Dong
Alternative: June 6 (3rd Floor
Exam Wed., June 27 1.00pm SR 3rd floor!
May 2
1. Cell clulture, protein expression and purification :
Primary cultures, organtypical cultures, differentiation models, eukaryotic expression systems, vector systems-Andreas Brachner
May 7
2.HS Struktur- Chemie B Protein-Protein, Protein DNA interactions:
In vitro binding assays, affinity (Scatchard), In situ analyses (IP), in vivo analyses, FRET, yeast two hybrid Bandshift, Footprinting, Chromatin-IP, Yeast one-hybrid Reporter Gene Assays-Andreas Brachner
May 9
3. Dynamics and Spatial Organisation of the Genome
Chromosome Conformation Capture techniques, GFP-tagging of chromosomes, High-resolution mapping ofgene territories-Alwin Köhler
May 14
4. Gene Silencing techniques RNA-Interferenz; Antisense, morpholino, epigenetics-Josef Gotzmann
May 16
5. Light Microscopy:
Immunofluorescence, wide field, confocal, live cell imaging, FRAP, FRET-Josef Gotzmann
May 21
6.
please don't turn beamer/pc off Ananlyses of cell cycle and Tumorbiology
Cell Cycle Stages (FACS, Elutriation, BrdU incorporation, proliferation marker, synchronization EMT, telomeres / DNA damage, transgenic and knock out tumor models-Roland Foisner
May 23
7. Proteomics:
Protein-identification (LC-MS/MS, mass spec, bio-informatics- Christopher Gerner
May 30
8. Techniques in Lipid Biochemistry- Sascha Martens
June 4
9. 3rd floor, seminarroom X-crystallography
Protein purification, Crystallisation, X-ray analyses, structure determination-Gang Dong
Alternative: June 6 (3rd Floor
Exam Wed., June 27 1.00pm SR 3rd floor!
Details
Language: English
Examination dates
Lecturers
- Roland Foisner
- Andreas Brachner
- Gang Dong
- Christopher Gerner
- Josef Gotzmann
- Alwin Köhler
- Sascha Martens
Classes
Currently no class schedule is known.
Information
Aims, contents and method of the course
The lecture deals with modern state of the art methods and applications in molecular cell biological research. It is designed for students familiar with the basic theory and basic practical approaches in cell and molecular biology. The taught subjects are adapted annually to allow incorporation of new developments in the field. Currenly the topics include: modern light and electron microscopical techniques, life cell imaging, organotypical cell culture, epigenetic analyses, RNAi approaches, analyses of gene expression, cell cycle regulation, protein-protein and protein DNA interactions, DNA microarray techniques and proteomic approaches.
Assessment and permitted materials
Minimum requirements and assessment criteria
Examination topics
Reading list
Association in the course directory
MMB III-2, MMB IV-2, MMB IV-3, MMB W-2, M202, M210
Last modified: Mo 07.09.2020 15:43