Universität Wien
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300484 UE Practical Course in Molecular Microbiology, Microbial Ecology and Immunobiology (2020W)

Diversity and function of uncultured microbes in medical and environmental samples

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 12 participants
Language: English

Lecturers

Classes (iCal) - next class is marked with N

This course will be held in-person (i.e. physical presence).
There will be a virtual Vorbesprechung on January 18 at 11 am.

  • Monday 01.02. 09:00 - 17:00 Übungsraum 6
    Übungsraum 7
  • Tuesday 02.02. 09:00 - 17:00 Übungsraum 6
    Übungsraum 7
  • Wednesday 03.02. 09:00 - 17:00 Übungsraum 6
    Übungsraum 7
  • Thursday 04.02. 09:00 - 17:00 Übungsraum 6
    Übungsraum 7
  • Friday 05.02. 09:00 - 17:00 Übungsraum 6
    Übungsraum 7
  • Monday 08.02. 09:00 - 17:00 Übungsraum 6
    Übungsraum 7
  • Tuesday 09.02. 09:00 - 17:00 Übungsraum 6
    Übungsraum 7
  • Wednesday 10.02. 09:00 - 17:00 Übungsraum 6
    Übungsraum 7
  • Thursday 11.02. 09:00 - 17:00 Übungsraum 6
    Übungsraum 7
  • Friday 12.02. 09:00 - 17:00 Übungsraum 6
    Übungsraum 7

Information

Aims, contents and method of the course

Course goals: In this course you will learn modern methods fur studying microorganisms without cultivation, including how to identify microorganisms and visualize them directly in environmental samples and how to study the activity and function of organisms in complex microbial communities.

Places in the course will be assigned based on the set points, the requirements of the different master programs and waiting time.

Assessment and permitted materials

Evaluation will have three parts: 1) practical work in the lab, 2) seminars, and 3) written report. Participants will need to pass all three evaluations to pass the course.

Minimum requirements and assessment criteria

Active participation in lab experiments and seminar (incl. presentation), as well as written protocol.

Examination topics

Methods: Theoretical knowledge will be disseminated through lectures (PowerPoint Presentations), the course handout and original literature. Supervised hands-on training in independent experiments will teach you how to identify microorganisms through 16S rRNA gene/transcript analysis and fluorescence in situ hybridization techniques (FISH/ CARD-FISH). You will further learn about the use of functional genes/transcripts to identify microorganisms involved in a certain function, and how to investigate their activity with stable isotope probing incubations. The theoretical principles of high-resolution secondary ion mass spectrometry (NanoSIMS) and Raman microspectroscopy coupled to stable isotope incubations will also be discussed.

The course includes computer-based sequence analysis as well as presentations and discussion of the results in student seminars.

Reading list

Association in the course directory

MMEI I, MEC-9

Last modified: Sa 22.10.2022 00:30