300517 UE+VO Theory and Application of Confocal Microscopy (2006W)

After rapid development, the Confocal Laser Scanning Microscope (CLSM) became a most important tool in both organismic and molecular biology. In this course, we present:
o the theoratical background of CLSM
o its application in structure analysis
o analysis and quantification of physiological parameters like membrane potential, ion concentration, diffusion of molecules, photosynthesis and respiration
in a hands-on training, we will apply the following techniques:
o localisation and identification of structures with organelle specific fluorescent dyes and antibodies
o 4D analysis
o ratio imaging
o 3D reconstruction and animation
o spectral dye separation
Participants are encouraged to bring their own samples. Positive examination of this lab course is a prerequisite for independent work on the CLSM of the Cell Imaging and Ultrastructure Research Unit.