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301046 UE Laboratory Course I B - Microbiology and Genetics (2024S)
Continuous assessment of course work
Labels
Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 08.02.2024 14:00 to Th 22.02.2024 18:00
- Deregistration possible until Th 22.02.2024 18:00
Registration information is available for each group.
Groups
Group 1
Eine Teilnahme an dieser Lehrveranstaltung ist erst nach Absolvierung einer Online-Sicherheitsunterweisung (diverse Module) möglich. Die Prüfung kann ab dem Tag nach einer erfolgreichen Anmeldung und Freischaltung zur Lehrveranstaltung in Moodle absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.-----
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.-----
max. 20 participants
Language: German, English
LMS: Moodle
Lecturers
- Boris Görke
- Michele Paganini (Student Tutor)
Classes (iCal) - next class is marked with N
see german version
- Monday 11.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 13.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 14.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 15.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Monday 18.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 19.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 20.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 21.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 22.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
Aims, contents and method of the course
Practical course in basic molecular biology techniques. Students will be introduced to the following techniques: DNA extraction from bacteria and yeast, DNA digests and ligations, transformation of bacteria, PCR, protein expression in bacteria, SDS-PAGE.
Assessment and permitted materials
The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the final exam must be passed with success).
Minimum requirements and assessment criteria
UPDATE: 09.03.2021:
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 33%; labbook and report (protocol): 33%; final written examination: 34%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
**************OLD VERSION BELOW***************
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 33%; labbook and report (protocol): 33%; final written examination: 34%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
**************OLD VERSION BELOW***************
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
Group 2
Eine Teilnahme an dieser Lehrveranstaltung ist erst nach Absolvierung einer Online-Sicherheitsunterweisung (diverse Module) möglich. Die Prüfung kann ab dem Tag nach einer erfolgreichen Anmeldung und Freischaltung zur Lehrveranstaltung in Moodle absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.-----
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.-----
max. 20 participants
Language: German, English
LMS: Moodle
Lecturers
- Katharina Semrad
- Ivan Yudushkin
- Tamara Civetta (Student Tutor)
Classes (iCal) - next class is marked with N
see german version
- Monday 11.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 13.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 14.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 15.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Monday 18.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 19.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 20.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 21.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 22.03. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
Aims, contents and method of the course
Practical course in basic molecular biology techniques. Students will be introduced to the following techniques: DNA extraction from bacteria and yeast, DNA digests and ligations, transformation of bacteria, PCR, protein expression in bacteria, SDS-PAGE.
Assessment and permitted materials
The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the final exam must be passed with success).
Minimum requirements and assessment criteria
UPDATE: 09.03.2021:
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 33%; labbook and report (protocol): 33%; final written examination: 34%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
**************OLD VERSION BELOW***************
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 33%; labbook and report (protocol): 33%; final written examination: 34%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
**************OLD VERSION BELOW***************
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
Group 3
Eine Teilnahme an dieser Lehrveranstaltung ist erst nach Absolvierung einer Online-Sicherheitsunterweisung (diverse Module) möglich. Die Prüfung kann ab dem Tag nach einer erfolgreichen Anmeldung und Freischaltung zur Lehrveranstaltung in Moodle absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.-----
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.-----
max. 20 participants
Language: German, English
LMS: Moodle
Lecturers
- Ivan Yudushkin
- Anna-Carolina Lenhart (Student Tutor)
Classes (iCal) - next class is marked with N
see german version
- Monday 08.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 09.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 10.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 11.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 12.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Monday 15.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 16.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 17.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 18.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 19.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
Aims, contents and method of the course
Practical course in basic molecular biology techniques. Students will be introduced to the following techniques: DNA extraction from bacteria and yeast, DNA digests and ligations, transformation of bacteria, PCR, protein expression in bacteria, SDS-PAGE.
Participation in the internship requires strict adherence to laboratory safety regulations. Gross disregard of lab safety rules will result in immediate expulsion from the practical course.
Participation in the internship requires strict adherence to laboratory safety regulations. Gross disregard of lab safety rules will result in immediate expulsion from the practical course.
Assessment and permitted materials
The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the final exam must be passed with success).
Participation in the practical course requires strict adherence to laboratory safety regulations. Gross disregard to the lab safety rules will result in immediate expulsion from the practical.
Participation in the practical course requires strict adherence to laboratory safety regulations. Gross disregard to the lab safety rules will result in immediate expulsion from the practical.
Minimum requirements and assessment criteria
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 33%; labbook and report (protocol): 33%; final written examination: 34%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
Group 4
Eine Teilnahme an dieser Lehrveranstaltung ist erst nach Absolvierung einer Online-Sicherheitsunterweisung (diverse Module) möglich. Die Prüfung kann ab dem Tag nach einer erfolgreichen Anmeldung und Freischaltung zur Lehrveranstaltung in Moodle absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.-----
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.-----
max. 20 participants
Language: German, English
LMS: Moodle
Lecturers
- Irma Querques
- Ivan Yudushkin
- Yelyzaveta Miller-Michlits (Student Tutor)
Classes (iCal) - next class is marked with N
see german version
- Monday 08.04. 08:00 - 11:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
- Monday 08.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 09.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 10.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 11.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 11.04. 13:15 - 14:45 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
- Friday 12.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Monday 15.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 16.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 17.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 17.04. 11:30 - 13:00 Seminarraum 1.5, Biologie Djerassiplatz 1, 1.012, Ebene 1
- Thursday 18.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 19.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
Aims, contents and method of the course
Practical course in basic molecular biology techniques. Students will be introduced to the following techniques: DNA extraction from bacteria and yeast, DNA digests and ligations, transformation of bacteria, PCR, protein expression in bacteria, SDS-PAGE.
Assessment and permitted materials
The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the final exam must be passed with success).
Minimum requirements and assessment criteria
UPDATE: 01.04.2024:
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 45%; lab book and report (protocol): 10%; final written examination: 45%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Lab book and report: following formal requirements of lab book keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.**************OLD VERSION BELOW***************
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 45%; lab book and report (protocol): 10%; final written examination: 45%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Lab book and report: following formal requirements of lab book keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.**************OLD VERSION BELOW***************
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
Group 5
Eine Teilnahme an dieser Lehrveranstaltung ist erst nach Absolvierung einer Online-Sicherheitsunterweisung (diverse Module) möglich. Die Prüfung kann ab dem Tag nach einer erfolgreichen Anmeldung und Freischaltung zur Lehrveranstaltung in Moodle absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.-----
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss bei Bedarf eine Laborbrille angeschafft werden. Privatsachen bitte in den Garderoberäumen verstauen.Participation in this course is only possible after completing an online safety instruction (various modules). The exam can be taken from the day after successful registration and activation of the course in Moodle. You can find out more at https://laborlehre-ubb.univie.ac.at/
All practice rooms may only be entered with a lab coat and closed shoes, also lab glasses must, if needed, be purchased. Please store private belongings in the cloakroom.-----
max. 20 participants
Language: German, English
LMS: Moodle
Lecturers
- Peter Schlögelhofer
- Giasemi-Angeliki Moraitou (Student Tutor)
Classes (iCal) - next class is marked with N
see german version
- Monday 22.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 23.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 24.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 25.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 26.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Monday 29.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 30.04. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 02.05. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 03.05. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
Aims, contents and method of the course
Practical course in basic molecular biology techniques. Students will be introduced to the following techniques: DNA extraction from bacteria and yeast, DNA digests and ligations, transformation of bacteria, PCR, protein expression in bacteria, SDS-PAGE.
Assessment and permitted materials
The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the final exam must be passed with success).
Minimum requirements and assessment criteria
UPDATE: 09.03.2021:
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 33%; labbook and report (protocol): 33%; final written examination: 34%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
**************OLD VERSION BELOW***************
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 33%; labbook and report (protocol): 33%; final written examination: 34%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
**************OLD VERSION BELOW***************
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
Information
Examination topics
Concentrations and dilutions, buffers, plasmids and their functional elements, plasmid extraction, PCR, restriction cloning, protein expression, SDS-PAGE, DNA (Sanger) sequencing. For methods used in the practical, it is essential to remember and understand the steps and their significance (what happens at each step). For buffers used during the practical, it is essential to understand the function of each component (remembering the exact concentration of each component is a plus, but not required).
Reading list
Recommended literature:
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
Association in the course directory
BMB 1, BMG 1, BBO 2
Last modified: We 31.07.2024 12:06