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301046 UE Laboratory Course I B - Microbiology and Genetics (2024W)
Continuous assessment of course work
Labels
Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 12.09.2024 14:00 to Th 26.09.2024 18:00
- Deregistration possible until Th 26.09.2024 18:00
Registration information is available for each group.
Groups
Group 1
Für diese LV wird eine verpflichtende Sicherheitsunterweisung stattfinden. Nach Ablauf der Anmeldephase und Aufnahme der Studierenden, muss diese absolviert werden. Der link zum entsprechenden Moodle-Kurs wird ihnen nach Aufnahme in den Kurs zugesendet.
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
_
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
_
max. 24 participants
Language: German, English
LMS: Moodle
Lecturers
Classes (iCal) - next class is marked with N
see german version
- Tuesday 01.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 02.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 03.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 04.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Monday 07.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 08.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 09.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 10.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 11.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
Minimum requirements and assessment criteria
a. Minimal requirement: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria:
- active participation 45%,
- protocol 10%,
- Theoretical basics and final exam 45% (all parts must passed positively, an opportunity for improvement in the case of negative exam results is possible).
b. Assessment criteria:
- active participation 45%,
- protocol 10%,
- Theoretical basics and final exam 45% (all parts must passed positively, an opportunity for improvement in the case of negative exam results is possible).
Examination topics
Concentrations and dilutions, buffers, plasmids and their functional elements, plasmid extraction, PCR, restriction cloning, protein expression, SDS-PAGE, DNA (Sanger) sequencing. For methods used in the practical course, it is essential to remember and understand the steps and their significance (what happens at each step). For buffers used during the practical, it is essential to understand the function of each component (remembering the exact concentration of each component is a plus, but not required).
The exam probes theoretical understanding of the taught exercises.
The exam probes theoretical understanding of the taught exercises.
Reading list
Recommended literature:
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
Group 2
Für diese LV wird eine verpflichtende Sicherheitsunterweisung stattfinden. Nach Ablauf der Anmeldephase und Aufnahme der Studierenden, muss diese absolviert werden. Der link zum entsprechenden Moodle-Kurs wird ihnen nach Aufnahme in den Kurs zugesendet.
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
_
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
_
max. 24 participants
Language: German, English
LMS: Moodle
Lecturers
Classes (iCal) - next class is marked with N
see german version
- Tuesday 01.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 01.10. 09:45 - 11:15 Seminarraum 1.6, Biologie Djerassiplatz 1, 1.011, Ebene 1
- Wednesday 02.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 03.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 04.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Monday 07.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 08.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 08.10. 09:45 - 13:00 Seminarraum 1.6, Biologie Djerassiplatz 1, 1.011, Ebene 1
- Wednesday 09.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 10.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 11.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
Minimum requirements and assessment criteria
UPDATE: 28.09.2023:
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 45%; lab book and report (protocol): 10%; final written examination: 45%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 45%; lab book and report (protocol): 10%; final written examination: 45%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics.
Examination topics
Concentrations and dilutions, buffers, plasmids and their functional elements, plasmid extraction, PCR, restriction cloning, protein expression, SDS-PAGE, DNA (Sanger) sequencing. For methods used in the practical, it is essential to remember and understand the steps and their significance (what happens at each step). For buffers used during the practical, it is essential to understand the function of each component (remembering the exact concentration of each component is a plus, but not required).
Reading list
Recommended literature:T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3 Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
Group 3
Für diese LV wird eine verpflichtende Sicherheitsunterweisung stattfinden. Nach Ablauf der Anmeldephase und Aufnahme der Studierenden, muss diese absolviert werden. Der link zum entsprechenden Moodle-Kurs wird ihnen nach Aufnahme in den Kurs zugesendet.
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
_
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
_
max. 24 participants
Language: German, English
LMS: Moodle
Lecturers
Classes (iCal) - next class is marked with N
see german version
- Monday 14.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 15.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 16.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 17.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 18.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Monday 21.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 22.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 23.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 24.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 25.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
Minimum requirements and assessment criteria
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
Examination topics
Concentrations and dilutions, buffers, plasmids and their functional elements, plasmid extraction, PCR, restriction cloning, protein expression, SDS-PAGE, DNA (Sanger) sequencing. For methods used in the practical, it is essential to remember and understand the steps and their significance (what happens at each step). For buffers used during the practical, it is essential to understand the function of each component (remembering the exact concentration of each component is a plus, but not required).
Reading list
Recommended literature:
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
Group 4
Für diese LV wird eine verpflichtende Sicherheitsunterweisung stattfinden. Nach Ablauf der Anmeldephase und Aufnahme der Studierenden, muss diese absolviert werden. Der link zum entsprechenden Moodle-Kurs wird ihnen nach Aufnahme in den Kurs zugesendet.
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
_
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
_
max. 24 participants
Language: German, English
LMS: Moodle
Lecturers
Classes (iCal) - next class is marked with N
see german version
- Monday 14.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 15.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 16.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 17.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 18.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Monday 21.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Tuesday 22.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Wednesday 23.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Thursday 24.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
- Friday 25.10. 09:00 - 17:00 Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
Minimum requirements and assessment criteria
a. Minimal requiremen: compulsory attendance, active participation, written protocol and final exam
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
b. Assessment criteria (key):
- active participation 50%,
- protocol 20%,
- Theoretical basics and final exam 30% (the final exam must be passed positively, an opportunity for improvement in the case of negative results is possible).
Examination topics
Concentrations and dilutions, buffers, plasmids and their functional elements, plasmid extraction, PCR, restriction cloning, protein expression, SDS-PAGE, DNA (Sanger) sequencing. For methods used in the practical, it is essential to remember and understand the steps and their significance (what happens at each step). For buffers used during the practical, it is essential to understand the function of each component (remembering the exact concentration of each component is a plus, but not required).
Reading list
Recommended literature:
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
T.A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th ed., Wiley Blackwell; ISBN 978-111907256-0
J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin's Genes XII (Chapters 1-3, 9-13, 17, 24), Jones & Bartlett Learning; ISBN 978-128410449-3
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, (ed. A. Hofmann, S. Clokie), Cambridge University Press; ISBN 978-131661476-1
Information
Aims, contents and method of the course
Practical course in basic molecular biology techniques. Students will be introduced to the following techniques: DNA extraction from bacteria and yeast, DNA digests and ligations, transformation of bacteria, PCR, protein expression in bacteria, SDS-PAGE.
Assessment and permitted materials
The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the final exam must be passed with success).
Association in the course directory
BMB 1, BMG 1 , BBO 2
Last modified: Tu 17.09.2024 16:06