Universität Wien
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301120 UE Practical Course in Molecular Genetics and Pathology (Cell Biology) (2022W)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
ON-SITE
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 16 participants
Language: German

Lecturers

Classes

Course requirements and admission criteria:
1. Attendance at the preliminary meeting (‘Vorbesprechung’);
2. Successfully completion of the lecture Zellbiologie tierischer Zellen" (Mikulits, Dolznig, Müllner) (SS), the Seminar "Seminar in Zellbiologie" can only be done together with this course!
3. Master’s students in A 066 877 Genetik und Entwicklungbiologiewill have preferential access;
4. Additional requirements for students who have not completed their bachelor's degree in the bachelor's program Biologie at the University of Vienna in the areas of BMG or BMB: External students who got practical courses (UE) as requirement by the admission to the Master´s program have to complete them in advance (bring a copy of your admission letter and a current transcript to the preliminary meeting).

Information

Aims, contents and method of the course

The practical course deals with 2-dimensional (2D) and 3-dimensional (3D) cell cultures of human carcinoma cells and carcinoma-associated fibroblasts. Experiments focus on the analysis of gene expression after RNA interference (RNAi), examination of the impact of conventional and targeted therapy on cell proliferation and clonogenic growth behavior in 2D and 3D cultures after generation of microspheres. In addition, the practical course concentrates on the analysis of hematopoiesis by flow cytometry, on the histology of various tissues and immunohistochemical analyses of experimental tumors after chemical induction and xenotransplantation of carcinoma cells.

Assessment and permitted materials

Evaluation of the experimental work and quality of the protocol.

Minimum requirements and assessment criteria

The students develop deeper and more comprehensive knowledge about the pathophysiology of tumorigenesis and its determinants. The students acquire technical skills and get access to the experimental methods of molecular cell biology to study the mechanisms of tumorigenesis.

Examination topics

2D and 3D cell cultures, generation of microspheres, RNAi/small interfering RNA, analysis of gene expression, invasion of spheres in collagen gels, treatment of 2D and 3D cell cultures with conventional and targeted chemotherapeutics, analysis of cell proliferation, clonogenicity and apoptosis after administration of chemotherapeutics, determination of cellular senescence, analysis of cell migration by woundhealing/scratch und Transwell assays, synchronization of the cell cycle, performance and interpretation of flow cytometry, histology and immunhistochemistry.

Reading list

Molecular Biology of the Cell, Bruce Alberts, Fifth Edition, 2008.
The Biology of Cancer, Robert A. Weinberg. 2007.
Rezente Übersichtsartikel zu respektiven Themen.

Association in the course directory

MMB III-2a:, MGE I-2, Biologische Chemie: BIO II-1,MGE III-1

Last modified: We 21.09.2022 14:28