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301151 UE Practical Course in fluorescence-/confocal microscopy including image processing (2024W)
for diploma, PhD and advanced students
Continuous assessment of course work
Labels
Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 12.09.2024 14:00 to Th 26.09.2024 18:00
- Deregistration possible until Th 26.09.2024 18:00
Registration information is available for each group.
Groups
Group 1
max. 12 participants
Language: German
LMS: Moodle
Lecturers
Classes
TEACHING LANGUAGE OF THE COURSE IS ENGLISH!!!!
PRELIMINARY MEETING (DIGITAL VIA ZOOM-see below): October, 7th, 2024; 10h30amATTENDANCE AT THE PRELIMINARY MEETING IS MANDATORY!!!!
Josef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Preliminary Meeting ConfCourse WS2024-25
Time: Oct 7, 2024 10:30 AM Vienna
Join Zoom Meeting: https://univienna.zoom.us/j/64153227619?pwd=WvcS0tl0yTbAvayDNnjy1bh2w3UbzE.1
Meeting ID: 641 5322 7619
Passcode: 413363Course details will be announced in Moodle: hhttps://moodle.univie.ac.at/course/view.php?id=433241!! REGISTRATION VIA U:SPACE FOR ONE OF THE OFFERED COURSES (301151-1 OR -2) IS SUFFICIENT!!
!! REGISTRATION VIA U:SPACE DOES NOT AUTOMATICALLY LEAD TO COURSE PARTICIPATION!IN CASE OF OVER-BOOKING THE ACTUAL SPL-RULES WILL BECOME APPLIED:
- The first 20 seats will be assigned to students of the Master-Program (865, 830, 834 and 877).
- Seat allocation will be performed according to the amount of the points bid.
- A quota-rule for the remaining 4 seats (2 seats/course) will become enacted for students from studies with a natural science background
- allocation of seats according to the amount of points bid
- @ points: if a vacant seat cannot become allocated based on points (e.g. same # of points bid), then the date of registration will decide (the earlier the better)Seat limitation: 12 (per course; in total for both courses 24 seats)Details for course program, schedule and workflow in the preliminary meeting.
Seat allocation will be done shortly (2-3 days) after the preliminary meeting.
Further details and information will then be distributed via Moodle.
Any changes will be announced in time!COURSE DATES: VALID FOR BOTH COURSES: November 11th-13th; 19th+20th; 26th+27th; DECEMBER 3rd+4th
An attendance at all these dates is MANDATORY!
Group 2
max. 12 participants
Language: German
LMS: Moodle
Lecturers
Classes
TEACHING LANGUAGE OF THE COURSE IS ENGLISH!!!!
PRELIMINARY MEETING (DIGITAL VIA ZOOM-see below): October, 7th, 2024; 10h30amATTENDANCE AT THE PRELIMINARY MEETING IS MANDATORY!!!!
Josef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Preliminary Meeting ConfCourse WS2024-25
Time: Oct 7, 2024 10:30 AM Vienna
Join Zoom Meeting: https://univienna.zoom.us/j/64153227619?pwd=WvcS0tl0yTbAvayDNnjy1bh2w3UbzE.1
Meeting ID: 641 5322 7619
Passcode: 413363Course details will be announced in Moodle: hhttps://moodle.univie.ac.at/course/view.php?id=433241!! REGISTRATION VIA U:SPACE FOR ONE OF THE OFFERED COURSES (301151-1 OR -2) IS SUFFICIENT!!
!! REGISTRATION VIA U:SPACE DOES NOT AUTOMATICALLY LEAD TO COURSE PARTICIPATION!IN CASE OF OVER-BOOKING THE ACTUAL SPL-RULES WILL BECOME APPLIED:
- The first 20 seats will be assigned to students of the Master-Program (865, 830, 834 and 877).
- Seat allocation will be performed according to the amount of the points bid.
- A quota-rule for the remaining 4 seats (2 seats/course) will become enacted for students from studies with a natural science background
- allocation of seats according to the amount of points bid
- @ points: if a vacant seat cannot become allocated based on points (e.g. same # of points bid), then the date of registration will decide (the earlier the better)Seat limitation: 12 (per course; in total for both courses 24 seats)Details for course program, schedule and workflow in the preliminary meeting.
Seat allocation will be done shortly (2-3 days) after the preliminary meeting.
Further details and information will then be distributed via Moodle.
Any changes will be announced in time!COURSE DATES: VALID FOR BOTH COURSES: November 11th-13th; 19th+20th; 26th+27th; DECEMBER 3rd+4th
An attendance at all these dates is MANDATORY!
Information
Aims, contents and method of the course
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, continuous assessment of theoretical knowledge (50%/20%/30%); each of the sub-grades must have a positive evaluation.
Minimum requirements and assessment criteria
The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.
Examination topics
microscopy sample preparation
theory of widefield fluorescence microscopy
measuring point spread function and chromatic aberration
theoretical and practical aspects of confocal microscopy
image processing
theory of widefield fluorescence microscopy
measuring point spread function and chromatic aberration
theoretical and practical aspects of confocal microscopy
image processing
Reading list
J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006
D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009
E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009
Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008
Molecular Biology of the Cell, Alberts, Garland Sciences,
Review series on „Imaging in Cell Biology“ in Nature Cell Biology Vol 5 (2003) Supplement
Review series on „Biological Imaging“ in Science Vol 300 (2003), 82-99
Eric C. Wait, Michael A. Reiche, Teng-Leong Chew: “Hypothesis-driven quantitative fluorescence microscopy – the importance of reverse-thinking in experimental design”, Journal of Cell Science 2020 133: jcs250027 doi: 10.1242/jcs.250027
Jonkman, J., Brown, C.M., Wright, G.D. et al. Guidance for quantitative confocal microscopy. Nat Protoc (2020). https://doi.org/10.1038/s41596-020-0307-7
https://www.ibiology.org/online-biology-courses/microscopy-series/
D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009
E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009
Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008
Molecular Biology of the Cell, Alberts, Garland Sciences,
Review series on „Imaging in Cell Biology“ in Nature Cell Biology Vol 5 (2003) Supplement
Review series on „Biological Imaging“ in Science Vol 300 (2003), 82-99
Eric C. Wait, Michael A. Reiche, Teng-Leong Chew: “Hypothesis-driven quantitative fluorescence microscopy – the importance of reverse-thinking in experimental design”, Journal of Cell Science 2020 133: jcs250027 doi: 10.1242/jcs.250027
Jonkman, J., Brown, C.M., Wright, G.D. et al. Guidance for quantitative confocal microscopy. Nat Protoc (2020). https://doi.org/10.1038/s41596-020-0307-7
https://www.ibiology.org/online-biology-courses/microscopy-series/
Association in the course directory
MMB III-1a:, MMB III-2a:, MMB III-3a:, MMB III-4a:, PhD, MMEI III
Last modified: Mo 16.09.2024 08:26
quality criteria and correction of aberrationsSELF-CONTAINED practical microscopy of selected samples by confocal microscopy (multichannel detection, serial optical sections through samples, image projections, generation of time series)2 days of Image Processing: Analysis and deconvolution of acquired images; Introduction into Image J