Warning! The directory is not yet complete and will be amended until the beginning of the term.
301171 UE Course III A - Laboratory Work in Molecular Biology (2020W)
for the specialization Microbiology/Genetics
Continuous assessment of course work
Labels
ON-SITE
Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 10.09.2020 08:00 to Th 24.09.2020 18:00
- Deregistration possible until Sa 31.10.2020 18:00
Registration information is available for each group.
Groups
Group 1
max. 16 participants
Language: German, English
LMS: Moodle
Lecturers
Classes (iCal) - next class is marked with N
Mandatory preliminary meeting: October 9, 11 a.m. to noon, via Zoom.
Date: November 16-27, 2020
Accompanying seminar 301172 mandatory!
Location: ÜS 1, Level 6, BZB, Dr. Bohrgasse 9, 1030 Vienna.
See also the student website of the departments: http://molekularebiologie.univie.ac.at/
- Friday 09.10. 11:00 - 12:00 Digital (Kickoff Class)
- Monday 16.11. 09:00 - 17:05 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Tuesday 17.11. 09:00 - 17:05 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Wednesday 18.11. 09:00 - 17:05 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Thursday 19.11. 09:00 - 17:05 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Friday 20.11. 09:00 - 17:05 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Monday 23.11. 09:00 - 17:05 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Tuesday 24.11. 09:00 - 17:05 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Wednesday 25.11. 09:00 - 17:05 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Thursday 26.11. 09:00 - 17:05 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Friday 27.11. 09:00 - 17:05 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: cloning of genes (including PCR amplification) and expression in E. coli, detection of the protein by western blot analysis. Isolation of a gene, labelling and use in Southern blot analysis.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
a. Minimum requirement: participation during the internship, writing a protocol, exam,
b. Assessment standard: participation 50%, minutes 30%, exam 20%
b. Assessment standard: participation 50%, minutes 30%, exam 20%
Examination topics
Content of the course held
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Group 2
max. 16 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
see german version
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: cloning of genes (including PCR amplification) and expression in E. coli, detection of the protein by western blot analysis. Isolation of a gene, labelling and use in Southern blot analysis.
Maximal number of participants: 20
Maximal number of participants: 20
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
a. Minimum requirement: participation during the internship, writing a protocol, exam,
b. Assessment standard: participation 50%, minutes 30%, exam 20%
b. Assessment standard: participation 50%, minutes 30%, exam 20%
Examination topics
Content of the course held
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Group 3
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
Mandatory preliminary meeting:
Tuesday 6th October, 10 - 11:30 a.m., via Jitsi Meet.
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Examination topics
Contents and theoretical background of the course units.
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Group 4
max. 16 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
see german version
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar:
e.g. Cloning and expression of certain genes in bacteria, yeast or plants including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern/Southern blotting; bacterial or yeast two-hybrid technology, expression analysis of reporter genes, expression of gfp (encoding green flourescence protein) and genes encoding gfp-fusion-proteinsMaximal number of participants: 16
e.g. Cloning and expression of certain genes in bacteria, yeast or plants including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern/Southern blotting; bacterial or yeast two-hybrid technology, expression analysis of reporter genes, expression of gfp (encoding green flourescence protein) and genes encoding gfp-fusion-proteinsMaximal number of participants: 16
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
Minimum requirements for passing the course: Attendance, protocol, final exam.Assessment criteria: participation 50%, protocol 30%, written exam 20%
Examination topics
Contents and theoretical background of the course units
Reading list
Current Literature and background information will be made available on Moodle
Association in the course directory
BMG 5
Last modified: Fr 12.05.2023 00:24