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301171 UE Course III A - Laboratory Work in Molecular Biology (2024W)
for the specialization Microbiology/Genetics
Continuous assessment of course work
Labels
Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 12.09.2024 14:00 to Th 26.09.2024 18:00
- Deregistration possible until Tu 15.10.2024 18:00
Registration information is available for each group.
Groups
Group 1
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes (iCal) - next class is marked with N
Mandatory preliminary meeting: Tuesday 1st October 2024 13:30h Seminar Room 1 Dr.-Bohr-Gasse 9/6.501-6.504
Lab course dates: 14.10. - 1.11.2024 whole dayPlace: Teaching Lab A Dr.-Bohr-Gasse 9/6.519 A-1030 ViennaAccompanying Seminar 301172 mandatory !Please see also department website for students: http://molekularebiologie.univie.ac.at/
- Tuesday 01.10. 13:30 - 14:30 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien (Kickoff Class)
Aims, contents and method of the course
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Examination topics
Content of the course held
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Group 2
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
Mandatory preliminary meeting: 2 October 2024, 10 a.m., seminar room 2, level 6, BZB, Dr. Bohrgasse 9, 1030 Vienna
Date: 4 - 22 November 2024 all day
Location: ÜR/BZB, Dr. Bohrgasse 9, 1030 Vienna
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Examination topics
Content of the course held
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Group 3
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes (iCal) - next class is marked with N
Verpflichtende Vorbesprechung am 2.10.2024 10:00 SR1
Termin: 4.- 22.11.2024 ganztägig
Ort: ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien
Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/
- Wednesday 02.10. 10:00 - 11:00 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien (Kickoff Class)
- Monday 04.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Tuesday 05.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Wednesday 06.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Thursday 07.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Friday 08.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Monday 11.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Tuesday 12.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Wednesday 13.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Thursday 14.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Friday 15.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Monday 18.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Tuesday 19.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Wednesday 20.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Thursday 21.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
- Friday 22.11. 09:00 - 17:00 BZB-Übungsraum 1A, 6.Ebene 6.519, Dr.-Bohrgasse 9, 1030 Wien
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar:
e.g. Cloning and expression of certain genes in bacteria including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern blotting; expression analysis of reporter genesMaximal number of participants: 18
e.g. Cloning and expression of certain genes in bacteria including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern blotting; expression analysis of reporter genesMaximal number of participants: 18
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
Minimum requirements for passing the course: Attendance, protocol, final exam.Assessment criteria: participation 50%, protocol 30%, written exam 20%
Examination topics
Contents and theoretical background of the course units.
Reading list
Current Literature and background information will be made available on Moodle
Group 4
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
CHANGE OF DATE: FR 27. Sept. 9:00-10:30 INFO MEETING CANCELLED!!
INSTEAD: MONDAY 30. SEPT. 6.th floor; 9:00, Übungsraum D
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: e.g. Cloning and expression of certain genes in bacteria, yeast or plants including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern/Southern blotting; bacterial or yeast two-hybrid technology, expression analysis of reporter genes, expression of gfp (encoding green flourescence protein) and genes encoding gfp-fusion-proteins Maximal number of participants: 16
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Examination topics
Contents and theoretical background of the course units
Reading list
Current Literature and background information will be made available on Moodle
Group 5
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes (iCal) - next class is marked with N
Mandatory preliminary meeting: Tuesday 1st October 2024 13:30h Seminar Room 1 Dr.Bohrgasse 9/6.501-6.504
Lab course dates: 25.11. - 13.12.2024 whole dayPlace: Teaching Lab D Dr.-Bohr-Gasse 9/6.526 A-1030 ViennaAccompanying Seminar 301172 mandatory !Please see also department website for students: http://molekularebiologie.univie.ac.at/
- Tuesday 01.10. 13:30 - 14:30 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Examination topics
Content of the course held
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Association in the course directory
BMG 5
Last modified: Sa 09.11.2024 17:06
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.