Universität Wien
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301171 UE Course III A - Laboratory Work in Molecular Biology (2025S)

10.00 ECTS (8.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 Schweighofer
2 Witte
4 Semrad
5 Schweighofer
6 Campbell

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

max. 18 participants
Language: German, English

Lecturers

Classes

Mandatory preliminary meeting:
Tuesday 4th March 2025 15:00h Seminar Room 1 Dr.-Bohr-Gasse 9/6.501-6.504

Lab course dates: 24.03. - 11.04.2025 whole day

Place: Teaching Lab A Dr.-Bohr-Gasse 9/6.519 A-1030 Vienna
Accompanying Seminar 301172 mandatory !

Please see also department website for students: http://molekularebiologie.univie.ac.at/

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Group 2

max. 18 participants
Language: German, English

Lecturers

Classes

Verpflichtende Vorbesprechung: Termin folgt
Termin: 19.5. - 6.6.2025 ganztägig
Ort: ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien

Begleitendes Seminar 301172 verpflichtend !

Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Group 3

max. 18 participants
Language: German, English

Lecturers

Classes (iCal) - next class is marked with N

Verpflichtende Vorbesprechung: 3.3.2025 10:00 SR1
Termin: 24.3.-11.4.2025 ganztägig
Begleitendes Seminar 301172 verpflichtend !

Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/

  • Monday 24.03. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Tuesday 25.03. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Wednesday 26.03. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Thursday 27.03. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Friday 28.03. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Monday 31.03. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Tuesday 01.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Wednesday 02.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Thursday 03.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Friday 04.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Monday 07.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Tuesday 08.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Wednesday 09.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Thursday 10.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
  • Friday 11.04. 09:00 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (20%)
+) verbal/written examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):

Permitted material for written exam: calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (20%)
+) verbal/written examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):

Group 4

max. 18 participants
Language: English

Lecturers

Classes

Verpflichtende Vorbesprechung: Termin folgt
Termin: 28.4. - 16.5.2025 ganztägig
Ort: ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien

Begleitendes Seminar 301172 verpflichtend !

Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Group 5

max. 18 participants
Language: German, English

Lecturers

Classes

Mandatory preliminary meeting:
Tuesday 4th March 2025 15:00h Seminar Room 1 Dr.-Bohr-Gasse 9/6.501-6.504

Lab course dates: 09.06. - 27.06.2025 whole day

Place: Teaching Lab A Dr.-Bohr-Gasse 9/6.519 A-1030 Vienna
Accompanying Seminar 301172 mandatory !

Please see also department website for students: http://molekularebiologie.univie.ac.at/

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Group 6

max. 18 participants
Language: German, English

Lecturers

Classes

Mandatory premeeting on 12.03.2025, 15:00, online:
https://univienna.zoom.us/j/69146852020?pwd=Xlsaz0sHYZdD6X2ReRLaF9XDDvmXxg.1
Course duration: 30.6. - 18.7.2025 all day
Ort: ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien

The accompanying Seminar 301172 is required !

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Information

Aims, contents and method of the course

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.

Examination topics

Contents and theoretical background of the course units.

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Association in the course directory

BMG 5

Last modified: Fr 17.01.2025 12:46