Universität Wien
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301484 UE Practical Course in Molecular Microbiology, Microbial Ecology and Immunobiology (2024W)

Diversity and function of uncultured microbes in medical and environmental samples

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Für diese LV wird eine verpflichtende Sicherheitsunterweisung stattfinden. Nach Ablauf der Anmeldephase und Aufnahme der Studierenden, muss diese absolviert werden. Der link zum entsprechenden Moodle-Kurs wird ihnen nach Aufnahme in den Kurs zugesendet.
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.

There will be a mandatory safty instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
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Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 14 participants
Language: English

Lecturers

Classes (iCal) - next class is marked with N

Preliminary meeting for this course will take place on Thursday, January 30th at 10 AM in Seminar Room 2.1. All participants must attend the preliminary meeting.

  • Tuesday 18.02. 08:00 - 18:15 Seminarraum 2.1, Biologie Djerassiplatz 1, 2.129, Ebene 2
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Wednesday 19.02. 08:00 - 18:15 Seminarraum 2.1, Biologie Djerassiplatz 1, 2.129, Ebene 2
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Thursday 20.02. 08:00 - 18:15 Seminarraum 2.1, Biologie Djerassiplatz 1, 2.129, Ebene 2
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Friday 21.02. 08:00 - 18:15 Seminarraum 2.1, Biologie Djerassiplatz 1, 2.129, Ebene 2
    Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Monday 24.02. 08:00 - 18:15 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Tuesday 25.02. 08:00 - 18:15 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Wednesday 26.02. 08:00 - 18:15 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Thursday 27.02. 08:00 - 18:15 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Friday 28.02. 08:00 - 18:15 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1

Information

Aims, contents and method of the course

Course goals: In this course you will learn modern methods fur studying microorganisms without cultivation, including how to identify microorganisms and visualize them directly in environmental samples and how to study the activity and function of organisms in complex microbial communities.

Places in the course will be assigned based on the set points, the requirements of the different master programs and waiting time.

Assessment and permitted materials

Evaluation will have three parts: 1) practical work in the lab, 2) seminars, and 3) written report. Participants will need to pass all three evaluations to pass the course.

Minimum requirements and assessment criteria

Active participation in lab experiments and seminar (incl. presentation), as well as written protocol.

Examination topics

Methods: Theoretical knowledge will be disseminated through lectures (PowerPoint Presentations), the course handout and original literature. Supervised hands-on training in independent experiments will teach you how to identify microorganisms through 16S rRNA gene/transcript analysis and fluorescence in situ hybridization techniques (FISH/ CARD-FISH). You will further learn about the use of functional genes/transcripts to identify microorganisms involved in a certain function, and how to investigate their activity with stable isotope probing incubations. The theoretical principles of high-resolution secondary ion mass spectrometry (NanoSIMS) and Raman microspectroscopy coupled to stable isotope incubations will also be discussed.

The course includes computer-based sequence analysis as well as presentations and discussion of the results in student seminars.

Reading list

Literature will be provided during the course.

Association in the course directory

MMEI I

Last modified: Tu 06.08.2024 11:26