Universität Wien
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301843 UE Practical Course in Molecular and Cellular Immunobiology (2024S)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 18 participants
Language: English

Lecturers

Classes

Preliminary meeting/Vorbesprechung: Tuesday, April 30, 13:00 – 14:00, 6th floor seminar room 1 (room 6.501). Attending the preliminary meeting is critical for securing a place in the course. If you cannot attend, please make sure to send a representative in your place.
Introductory lectures: Friday June 7, 2024, 12:30-16:30, 6th floor seminar room 3 (room 6.105)
Course: Monday, June 10 – Friday, June 28, 2024, 9.00 - 17.00h, Übungsräume/course rooms 6th floor, Max Perutz Labs, BZB, Dr.Bohrgasse 9, 1030 Wien
Exam: Thursday July 4, 12:30 - 14:00, 6th floor seminar room 1 (room 6.501), Max Perutz Labs, BZB, Dr.Bohrgasse 9, 1030 Wien

Curricular requirements for enrollment to the course for all participants: Completion of Lecture 301364 (Research topics in Molecular Microbiology, Microbial Ecology and Immunobiology).;Course requirements and admission criteria:
1. Attendance at the preliminary meeting (‘Vorbesprechung’);
2. Master’s students in A 066 830 'Molecular Microbiology, Microbial Ecology and Immunobiology' will have preferential access;
3. Additional requirements for students who have not completed their bachelor's degree in the bachelor's program Biologie at the University of Vienna in the areas of BMG or BMB: External students who got practical courses (UE) as requirement by the admission to the Master´s program have to complete them in advance (bring a copy of your admission letter and a current transcript to the preliminary meeting).
4. Students from other disciplines must have completed an introductory course in immunology (e.g., Immunology and Cellular Microbiology A).

Information

Aims, contents and method of the course

Maximum number of students accepted is 18, divided into groups of 3. The course takes three weeks in total.
a. Aims: Students should learn immunological techniques, e.g. working with cell culture (sterile handling of cell lines, transfection of cells, isolation and differentiation of primary cells from bone marrow and spleen), antiviral assays, fluorescence microscopy, luciferase assays, FACS, purification of antibodies, immune precipitation, Western blot. Acquiring knowledge and experimental skills concerning the innate immune system, and functional assays on genetically modified mammalian cells.
b. contents: In this course part we will use CRISPR-Cas9 technology to make knock-outs of key genes of the innate immune system in human cells. The generated cells will be functionally tested using reporter assays.

Assessment and permitted materials

Students are assessed on the following criteria:1) Performance during the course (practical skills, knowledge and reasoning during black-board discussion, initiative and participation).2) Written exam; tests for knowledge, ability to apply knowledge, and perform scientific calculations relevant to the course material.3) Written report in scientific manuscript format; includes quality of the data, and experimental outcome.During the practical days of the course, use of the course manual and calculators is allowed. During the written exam, only the use of a standard calculator is allowed.

Minimum requirements and assessment criteria

a. Minimum requirements: Compulsory attendance (attendance during the whole course is a minimum requirement); the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam. All students must have familiarized themselves with the course manual before start of the practical work.b. Assessment criteria:Example for a course with continuous assessment: You can achieve 100 points1) Performance during the course: 45 points.2) Written exam: 30 points.3) Written report, and data quality/ experimental outcome: 25 pointsGrading example: 1 (excellent) 100 90 points 2 (good) 89 81 points 3 (satisfactory) 80 71 points 4 (sufficient) 70 - 61 points 5 (insufficient) 60 0 points

Examination topics

- All information in the course manual - All information provided in non-written form during daily ‘blackboard’ teaching during the course; includes discussions of data interpretation and expected outcomes of experiments. - Scientific calculations relevant to the course material. - All information from introductory lectures

Reading list

The provided course manuals.

Association in the course directory

MMEI I, MMB III-2a:, MMB III-3a:, MMB III-4a:, BIO II-2

Last modified: Th 18.04.2024 09:26