Universität Wien
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301901 VO+UE Introduction into 3D cryo electron microscopy (2023W)

3.00 ECTS (2.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
ON-SITE
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 10 participants
Language: English

Lecturers

Classes (iCal) - next class is marked with N

see german version

  • Tuesday 21.11. 15:00 - 18:15 Hörsaal 3, Biologie Djerassiplatz 1, 0.005, Ebene 0
  • Wednesday 22.11. 08:00 - 11:15 Hörsaal 3, Biologie Djerassiplatz 1, 0.005, Ebene 0
  • Friday 24.11. 08:00 - 11:15 Hörsaal 3, Biologie Djerassiplatz 1, 0.005, Ebene 0
  • Friday 24.11. 09:45 - 13:00 Seminarraum 1.6, Biologie Djerassiplatz 1, 1.011, Ebene 1

Information

Aims, contents and method of the course

Mathematical Background Fourier Theory for 3D EM Correlation and Convolution Information theory for 3D EM Optics of the Transmission electron microscope The electron source The condenser system The objective and image generation within an electron microscope Electron detection Sample preparation Image processing Single particle 2D analysis Single particle 3D generation Single particle refinement and validation Tomographic processing Modern conceptsPracticals/Tutorials: Negative staining EM Cryo EM Tomographic acquisition Single particle image processing Tomographic image processing

Assessment and permitted materials

The evaluation consists of different performances: active participation and results obtained, theoretical knowledge including final exam.

Minimum requirements and assessment criteria

Compulsory attendance;A basic understanding of the concepts presented should be acquired.Active participation and results obtained, Theoretical knowledge (final exam);(The percentage of the subgrades will be announced by the course leader, each of the subgrades must have a positive evaluation.)

Examination topics

Content of the course units

Reading list

The lecturers will provide slides that gather the required material. Suggestions for further reading material will be provided during the lecture.

Association in the course directory

PhD (SPL 56), WZB, MMB W-2, MMEI III

Last modified: Fr 29.09.2023 15:48