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320113 VO Cell culture models in Pharmaceutical Technology (2014S)
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Termine: 29.4., 30.4., 6.5., 8.5., 9.5., 13.5., 15.5., 16.5., 20.5., 22.5., 23.5., 27.5., 28.5., 3.6., 5.6., 6.6. & 13.6.2014; jeweils 9.00-10.30 Uhr; UZA2, Raum 2E411
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Language: German
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Prerequisites: none
Examination: oral
Examination: oral
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A400
Last modified: We 19.08.2020 08:09
to give detailed information about the principles und application of pharmaceutical cell culture models, to establish a basis for self-dependent cell culture work
Contents:
basic characteristics of the mammaliancell (structure, molecular organisation, physiology) cell culture models in pharmaceutical analysis, suitable cell lines (Caco-2, HT29, ...- characteristics, special features, application) guidelines for cultivation of cells (materials and methods, practical application, precautions), tissue-models (preparation of monolayers, differentiation, trans-well-systems, TEER-measurements, marker compounds) application of pharmaceutical cell cultures (potentials, limits, comparison between in vitro and in vivo - systems), experimental setup (single cells, monolayers, trans-well-systems) analytical techniques using cell cultures: proliferation studies (MTT, BrdU), viability tests (trypane blue, ethidium bromide), fluorescence-reader, flow-cytometry, confocal laser scanning microscopy selected examples for the pharmaceutical application of Caco-2 cells (binding studies, competitive inhibition of cellular interactions, differences single cell/monolayer, transport studies)Prerequisites: none
Examination: oral