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580012 VU Introduction to method development and validation for LCMS/MS qualitative and quantitative analysis (2022W)
1.00 ECTS (1.00 SWS), SPL 58 - Doktoratsstudium Pharmazie, Ernährungswissenschaften und Sportwissensch
Continuous assessment of course work
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Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Mo 29.08.2022 13:10 to Mo 19.12.2022 12:00
- Deregistration possible until Mo 19.12.2022 12:00
Details
Language: German
Lecturers
Classes
The course will be offered in English or German, depending on the audience.
January 2023: KW 3 and KW 4Exact dates be announced in November!
Information
Aims, contents and method of the course
This course endows an introduction to the crucial notions and approaches in the method development and validation for LC–MSMS qualitative and quantitative analysis. Prominence will be shed on development of efficient, straight forward and universal methods used in common LC-MS Analysis. The aim of the course is to coach the students how to develop efficient LC methods, couple them to MS methods, understand, optimize and tune ESI source parameters. The course addresses various problems confronted when perusing studies/carrier in the field of MS oriented analytical chemistry. The course includes case studies in the fields of LC-MS analysis and small molecules analyses (OMICS).
Assessment and permitted materials
• Understand, optimize and tune LC parameters
• Understand, optimize and tune ESI source parameters
• Understand, optimize and tune MS experiments parameters
• Develop an outline of the key procedures and tools that are used in MS data analysis
• Be capable of developing at least: one untargeted experiment (in omics oriented fashion) and one targeted experiment (in 3Q absolute quantification fashion)
• Understand, optimize and tune ESI source parameters
• Understand, optimize and tune MS experiments parameters
• Develop an outline of the key procedures and tools that are used in MS data analysis
• Be capable of developing at least: one untargeted experiment (in omics oriented fashion) and one targeted experiment (in 3Q absolute quantification fashion)
Minimum requirements and assessment criteria
•Windows Laptop
Examination topics
Grade will be given based on three aspects:
1. Final evaluation based on self-developed methods
2. Lab assignments
1. Final evaluation based on self-developed methods
2. Lab assignments
Reading list
Association in the course directory
Last modified: Mo 26.09.2022 15:50