Universität Wien
Course Exam

301234 VO Advanced Biochemical and Biophysical Methods (2020S)

3.00 ECTS (2.00 SWS), SPL 30 - Biologie

Wednesday 03.06.2020 10:00 - 12:30 Digital
10.00 - 12.30 Uhr, open book online exam

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Examiners

Information

Examination topics

Protein and amino acid properties (pKa, pI, etc)
Methods of protein expression and purification (size exclusion chromatography, affinity chromatography, hydrophobic interaction- or ion exchange chromatography, purification tags, etc)
Methods of protein quality assessment and control
Basic principles of X-ray crystallography
Basic principles of SAXS, SLS and DLS
Basic principles of absorption and emission (fluorescence) spectroscopy (quantum yield, excitation/emission spectra, Stokes shift, Lambert-Baer's law, CD spectroscopy, etc)
Fluorescence correlation spectroscopy
Basic principles of NMR spectroscopy
Basic principles of mass spectrometry
Basic principles of digital light microscopy (contrast, resolution, magnification, electronic detection of light, binning, factors determining FRET efficiency, etc)
Basic principles of electron microscopy

Assessment and permitted materials

Written exam at the end of the lecture series. In the exam, you can achieve up to 100 percentage points.

Minimum requirements and assessment criteria

To attain the pass grade, more than 44.99% points are necessary
Grading scheme:
1 (excellent): 85-100%
2 (good): 75-84.99%
3 (satisfactory): 65-74.99%
4 (sufficient): 45-64.99%
5 (insufficient): 0-44.99%

Last modified: Fr 12.05.2023 00:24